Volume 20, Issue 2 (Summer 2023)                   Sci J Iran Blood Transfus Organ 2023, 20(2): 123-133 | Back to browse issues page

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Alavi P, Chegini A, Samiee S, Alaie M. Changes in the amount of mitochondrial DNA during storage in RBCs and Leukoreduced red blood cells. Sci J Iran Blood Transfus Organ 2023; 20 (2) :123-133
URL: http://bloodjournal.ir/article-1-1444-en.html
Changes in the amount of mitochondrial DNA during storage in RBCs and Leukoreduced red blood cells
P. Alavi , A. Chegini * , Sh. Samiee , M. Alaie
Keywords: Key words: RBCs, Mitochondrial, Genes
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    References:
  1. Bansod PN, Jethani N, Pachori G. Clinical use of blood and its components in tertiary health care center in northwestern India. Int J Med Sci Public Health 2015; 4: 787-91.
  2. Sahu S, Hemlata AV. Adverse events related to blood transfusion. Indian J Anaesth 2014; 58(5): 543-51.
  3. Toy P,  Popovsky  MA,   Abraham   E,   Ambruso DR,
  4. Holness LG, Kopko PM, et al. Transfusion-related acute lung injury: definition and review. Crit Care Med 2005; 33(4): 721-6.
  5. Silliman CC, Boshkov LK, Mehdizadehkashi Z, Elzi DJ, Dickey WO, Podlosky L, et al. Transfusion-related acute lung injury: epidemiology and a prospective analysis of etiologic factors. Blood 2003; 101(2): 454-62.
  6. Jy W, Ricci M, Shariatmadar S, Gomez-Marin O, Horstman LH, Ahn YS. Microparticles in stored red blood cells as potential mediators of transfusion complications. Transfusion 2011; 51(4): 886-93.
  7. Grazzini G, Vaglio S. Red blood cell storage lesion and adverse clinical outcomes: post hoc ergo propter hoc? Blood Transfus 2012; 10(Suppl 2): s4-6.
  8. Chen GY, Nuñez G. Sterile inflammation: sensing and reacting to damage. Nat Rev Immunol 2010; 10(12): 826-37.
  9. Vénéreau E, Ceriotti C, Bianchi ME. DAMPs from cell death to new life. Front Immunol 2015; 6: 422.
  10. Larsson NG. Somatic mitochondrial DNA mutations in mammalian aging. Annu Rev Biochem 2010; 79: 683-706.
  11. Krysko DV, Agostinis P, Krysko O, Garg AD, Bachert C, Lambrecht BN, et al. Emerging role of damage-associated molecular patterns derived from mitochondria in inflammation. Trends Immunol 2011; 32(4): 157-64.
  12. Simmon JD, Lee Y-L, Mulekar S, Kuck JL, Brevard SB, Gonzalez RP, et al. Elevated levels of plasma mitochondrial DNA DAMPs are linked to clinical outcome in severely injured human subjects. Ann Surg 2013; 258(4): 591-6.
  13. Zhang Q, Raoof M, Chen Y, Sumi Y, Sursal T, Junger W, et al. Circulating mitochondrial DAMPs cause inflammatory responses to injury. Nature 2010; 464(7285): 104-7.
  14. Nakahira K, Kyung SY, Rogers AJ, Gazourian L, Youn S, Massaro AF, et al. Circulating mitochondrial DNA in patients in the ICU as a marker of mortality: derivation and validation. PLoS Med 2013; 10(12): e1001577.
  15. Sun S, Sursal T, Adibnia Y, Zhao C, Zheng Y, Li H, et al. Mitochondrial DAMPs increase endothelial permeability through neutrophil dependent and independent pathways. PloS One 2013; 8(3): e59989.
  16. Bakkour S, Acker JP, Chafets DM, Inglis HC, Norris PJ, Lee TH, et al. Manufacturing method affects mitochondrial DNA release and extracellular vesicle composition in stored red blood cells. Vox Sang 2016; 111(1): 22-32.
  17. Tanji N, Tanji K, Kambham N, Markowitz GS, Bell A, D'Agati VD. Adefovir nephrotoxicity: possible role of mitochondrial DNA depletion. Hum Pathol 2001; 32(7): 734-40.
  18. Nakashima-Kamimura N, Asoh S, Ishibashi Y, Mukai Y, Shidara Y, Oda H, et al. MIDAS/GPP34, a nuclear gene product, regulates total mitochondrial mass in response to mitochondrial dysfunction.  J Cell Sci 2005; 118(22): 5357-67.
  19. Lepelley A, Crow YJ. Erythrocyte-derived mitochondria take to the lupus stage. Cell Metab 2021; 33(9): 1723-5.
  20. Lee Y-L, King MB, Gonzalez RP, Brevard SB, Frotan MA, Gillespie MN, et al. Blood transfusion products contain mitochondrial DNA damage-associated molecular patterns: a potential effector of transfusion-related acute lung injury. J Surg Res 2014; 191(2): 286-9.
  21. Simmons JD, Lee Y-LL, Pastukh VM, Capley G, Muscat CA, Muscat DC, et al. Potential contribution of mitochondrial DNA damage associated molecular patterns in transfusion products to the development of acute respiratory distress syndrome after multiple transfusions. J Trauma Acute Care Surg 2017; 82(6): 1023-9.
  22. Shih AW, Bhagirath VC, Heddle NM, Acker JP, Liu Y, Eikelboom JW, et al. Quantification of Cell-Free DNA in Red Blood Cell Units in Different Whole Blood Processing Methods. J Blood Transfus 2016; 2016: 9316385.
  23. Yasui K, Matsuyama N, Kuroishi A, Tani Y, Furuta RA, Hirayama F. Mitochondrial damage-associated molecular patterns as potential proinflammatory mediators in post-platelet transfusion adverse effects. Transfusion 2016; 56(5): 1201-12.
  24. Dijkstra-Tiekstra MJ, van der Schoot CE, Pietersz RN, Reesink HW. White blood cell fragments in platelet concentrates prepared by the platelet-rich plasma or buffy-coat methods. Vox Sang 2005; 88(4): 275-7.
  25. McQuinn ER, Smith SA, Viall AK, Wang C, LeVine DN. Neutrophil extracellular traps in stored canine red blood cell units. J Vet Intern Med 2020; 34(5): 1894-902.
  26. Cognasse F, Aloui C, Anh Nguyen K, Hamzeh-Cognasse H, Fagan J, Arthaud CA, et al. Platelet components associated with adverse reactions: predictive value of mitochondrial DNA relative to biological response modifiers. Transfusion 2016; 56(2): 497-504.







Sci J Iran Blood Transfus Organ 2023;20 (2): 123-133
Orginal Article
 



Changes in the amount of mitochondrial DNA during
 storage in RBCs and Leukoreduced red blood cells

Alavi P.1, Chegini A.1, Samiee Sh.1, Alaie M.1


1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran


Abstract
Background and Objectives
The mitochondrial DNA in blood products can cause physiological changes in cells and patient complications. This study aims to evaluate the effect of storage time on the copy number of free mitochondrial DNA in products of RBCs and leukoreduced red blood cells.

Materials and Methods
In a cross-sectional descriptive study, 15 units of RBCs and 15 units of leukoreduced red blood cells were evaluated. After determining the hematological parameters and the average blood cell count in each bag, the free mitochondrial DNA copy number was evaluated using the RT-PCR method. The mitochondrial DNA copy number was measured in blood products on days 0, 5 and 35. After data collection, Shapiro and Mann-Whitney tests were performed and the analysis was done using REST 2009 software.

Results
The average hemoglobin level in the blood bags was 17.6 ± 4.3 gr/dL. The mean platelet count in packed RBC bags was 132.02 ± 50.60´1000/mm3 of blood. The mean number of red blood cells was 6.07 ± 0.14 million per microliter of blood and the mean WBC count in the blood bags was 4.98 ± 2.3 per microliter. The number of free mitochondrial DNA copies in the packed RBC product was higher compared to the leukocyte-less RBC products on days 5 and 35.

Conclusions 
The free mitochondrial DNA copy number in RBCs bags was higher than leukoreduced RBCs,
and a direct relationship between the amount of WBC and the number of free mtDNA copy number was observed.

Key words: RBCs, Mitochondrial, Genes






Received:    6 Mar 2022
Accepted: 22 May 2023



Correspondence: Chegini A., MD. Specialist in Anesthesiology. Assistant Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 82052256; Fax: (+9821) 88601599
E-mail: a.chegini@ibto.ir
 
Type of Study: Research | Subject: Blood banking
Published: 2023/07/1
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