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Sci J Iran Blood Transfus Organ 2019, 16(3): 172-185 Back to browse issues page
Stem Cell Bone Differentiation on Polyol Lactic Acid Composite Nanoparticles Containing 45S5 Bioactive Glass Nanoparticles
M. Shams, R. Halabian Dr., M. Karimi Dr., M. Ghollasi Dr., A. Salimi ِDr.
Keywords: Key words: Nanocomposites, Stem Cells, Glass, Bone Marrow
Full-Text [PDF 881 kb]   (557 Downloads)     |   Abstract (HTML)  (1734 Views)
Type of Study: Research | Subject: Stem cells
Published: 2019/10/2
Full-Text:   (229 Views)
    References:
 
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Sci J Iran Blood Transfus Organ 2019;16 (3): 172-185
Original Article
 

 

Stem Cell Bone Differentiation on Polyol Lactic
Acid Composite Nanoparticles Containing 45S5 Bioactive
Glass Nanoparticles
 
Shams M.1, Halabian R.2, Karimi M.1, Ghollasi M.3, Salimi A.4
 
1Nanotechnology and Advanced Materials Institute, Materials and Energy Research Center(MERC), Karaj, Iran
2Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
3Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
4Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
 
Abstract
Background and Objectives
Now day, using of stem cells and nanoparticles in the differentiation of stem cells is considered as a therapeutic approach. The purpose of this study was to synthesize and characterize nanocomposite polyacrylic polycarboxylic acid containing nanoparticles of biologically active glass 45S5 crushed and assessment effect of this composite on the propagation and growth of bone marrow derived mesenchymal stem cells in the external environment.
 
Materials and Methods
In this experimental study, the bioactive glass nanoparticles were synthesized by planetary milling and coated on electrospun polyolactic acid nanofibers. The physicochemical and biological properties of the composite nanocarbon were evaluated by X-ray diffraction, scanning electron microscopy, MTT, acridine orange, and alkaline phosphatase assays.
 
Results
The physicochemical properties showed that the structure of synthesis bioactive glass and the lactic acid poly lactic acid fibers were completely nano size and the uniform distribution of nanoparticles was well placed on the fiber. The cell analysis demonstrated the significant increase proliferation and differentiation of mesenchymal stem cells to bone cells.In the MTT assay, cell survival was expressed in the composite nanostructure after 7 days of cell culture (1.96 ± 0.2), while the control sample (0.76 ± 0.08).
 
Conclusions 
According to the results of this study, the composite nanoscale composite is not cytotoxicity effects and has biocompatible. It is useful in bone tissue repair.
 
Key words: Nanocomposites, Stem Cells, Glass, Bone Marrow
 
 
 
 
 
 
Received:  10 Apr 2019
Accepted:   6 Jul  2019
 
 

Correspondence: Salimi A., PhD of Nanotechnology. Assistant Professor of Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences.    
P.O.Box: 14359-44711, Tehran, Iran. Tel: (+9821) 81263155; Fax: (+9821) 81263155
E-mail: salimibio@gmail.com
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Shams M, Halabian R, Karimi M, Ghollasi M, Salimi A. Stem Cell Bone Differentiation on Polyol Lactic Acid Composite Nanoparticles Containing 45S5 Bioactive Glass Nanoparticles. Sci J Iran Blood Transfus Organ. 2019; 16 (3) :172-185
URL: http://bloodjournal.ir/article-1-1261-en.html


Volume 16, Issue 3 (Autumn 2019) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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