Volume 15, Issue 4 (Winter 2018)                   Sci J Iran Blood Transfus Organ 2018, 15(4): 293-300 | Back to browse issues page

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Ahmadzadeh Shad G, Zadsar M, Shayegan M, Samiee S, Zare A. HPA-1 gene polymorphism in Iranian couples with history of recurrent abortion. Sci J Iran Blood Transfus Organ 2018; 15 (4) :293-300
URL: http://bloodjournal.ir/article-1-1215-en.html
HPA-1 gene polymorphism in Iranian couples with history of recurrent abortion
Gh. Ahmadzadeh Shad , M. Zadsar * , M. Shayegan , Sh. Samiee , A. Zare
Keywords: Key words: Platelets, Abortion, Recurrent, Thrombocytopenia
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Sci J Iran Blood Transfus Organ 2019; 15 (4): 293-300
Original Article
 

 

HPA-1 gene polymorphism in Iranian couples with
history of recurrent abortion
 
Ahmadzadeh Shad Gh.1,  Zadsar M.1, Shaiegan M.1, Samiee Sh.1, Zare A.2
 
 
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
2Sarem Fertility and Infertility Research Center, Sarem Cell Research Center, Sarem Hospital, Tehran, Iran
 
 
 
Abstract
Background and Objectives
Recurrent abortion is referred to a condition that 2 or 3 consecutive pregnancies are affected by abortions before the 20th week of gestation, which is likely to occur in 2-5% of pregnancies. There are some etiologic factors such as anatomical, infectious and immunological elements. One of these immunological factors is the polymorphism of the HPA-1 gene as a factor able to cause FNAIT and its related complications; besides it contributes to abortion as a thrombophilic gene. This study was conducted to evaluate the prevalence of HPA-1 incompatibility in Iranian couples with a history of recurrent abortions.
 
Materials and Methods
In this cross-sectional descriptive study, totally 75 couples with at least two recurrent pregnancy losses without any specified causes (according to the medical records obtained from the infertility center) were entered to the study. Four-ml blood samples were collected in EDTA tubes and the allelic frequency of HPA-1 was determined by PCR-SSP. Gene frequency was calculated using the Hardy Weinberg equation. The  p< 0.05 was considered significant.
 
Results
Totally 75 couples were included with the average age of women being 32 ± 7 years and the mean number of abortions 2.5 ± 0.9. HPA-1a was found in 100% of the under study  population, and HPA-1b was absent in this population.
 
Conclusions 
This study revealed that HPA-1a gene polymorphism does not have the likelihood of being involved in the recurrent abortion in Iranian couples, and other factors need to be investigated to determine the possible causes of recurrent abortion.
 
Key words: Platelets, Abortion، Recurrent, Thrombocytopenia
 
 
 
 
Received: 27 Jun 2018
Accepted:20 Aug 2018
 
 

Correspondence: Zadsar M., MD. Pathologist. Assistant Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 82052234; Fax: (+9821) 88628741
E-mail:
maryam.zad@gmail.com
Type of Study: Research | Subject: Imunohematology
Published: 2019/01/5
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