Volume 14, Issue 2 (Summer 2017)                   bloodj 2017, 14(2): 92-100 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Abasalizadeh M, Nehzati P, Allahbakhshian M, Hamidpour M, Khadem Mabudi A A, Tabatabaie M R. The detection of Scavenger Receptor-B1 expression and its role on the function of platelets in patients with atherosclerotic disease . bloodj 2017; 14 (2) :92-100
URL: http://bloodjournal.ir/article-1-1083-en.html
The detection of Scavenger Receptor-B1 expression and its role on the function of platelets in patients with atherosclerotic disease
Mahnaz Abasalizadeh , Prisa Nehzati , Mehdi Allahbakhshian , Mohsen Hamidpour * , Ali Akbar Khadem Mabudi , Mohamad Reza Tabatabaie
Keywords: Key words: Platelets, Atherosclerosis, Patients
Full-Text [PDF 1963 kb]   (2744 Downloads)     |   Abstract (HTML)  (5829 Views)
Full-Text:   (2598 Views)
References:
 
 
  1. Douglas G, Channon KM. The pathogenesis of atherosclerosis. Medicine 2014; 42(9): 480-4.
  2. Wang T, Palucci D, Law K, Yanagawa B, Yam J, Butany J. Atherosclerosis: pathogenesis and pathology. Diagnostic Histopathology 2012; 18(11): 461-7.
  3. Lloyd-Jones DM. Cardiovascular risk prediction basic concepts, current status, and future directions. Circulation 2010; 121(15): 1768-77.
  4. Lewis GF, Rader DJ. New insights into the regulation of  HDL  metabolism and reverse cholesterol transport.
Circ Res 2005; 96(12): 1221-32.
  1. Mineo C, Deguchi H, Griffin JH, Shaul PW. Endothelial and antithrombotic actions of HDL. Circ Res 2006; 98(11): 1352-64.
  2. Murphy AJ, Woollard KJ. High-density lipoprotein: a potent inhibitor of inflammation. Clin Exp Pharmacol Physiol 2010; 37(7): 710-8.
  3. Kent AP, Stylianou IM. Scavenger receptor class B member 1 protein: hepatic regulation and its effects on lipids    ,     reverse     cholesterol      transport   ,      and
atherosclerosis. Hepat Med 2011; 3: 29-44.
  1. Luo DX, Cao DL, Xiong Y, Peng XH, Liao DF. A novel model of cholesterol efflux from lipid-loaded cells. Acta Pharmacol Sin 2010; 31(10): 1243-57.
  2. Gordon DJ, Rifkind BM. High-density lipoprotein--the clinical implications of recent studies. N Engl J Med 1989; 321(19): 1311-6.
  3. von Eckardstein A, Nofer JR, Assmann G. High density lipoproteins and arteriosclerosis role of cholesterol efflux and reverse cholesterol transport. Arterioscler Thromb Vasc Biol 2001; 21(1): 13-27.
  4. Mineo C, Shaul PW. Functions of scavenger receptor class B, type I in atherosclerosis. Curr Opin Lipidol 2012; 23(5): 487-93.
  5. Yancey PG, Bortnick AE, Kellner-Weibel G, de la Llera-Moya M, Phillips MC, Rothblat GH. Importance of different pathways of cellular cholesterol efflux. Arterioscler Thromb Vasc Biol 2003; 23(5): 712-9.
  6. Shen WJ, Hu J, Hu Z, Kraemer FB, Azhar S. Scavenger receptor class B type I (SR-B1): a versatile receptor with multiple functions and actions. Metabolism 2014; 63(7): 875-86.
  7. Valiyaveettil M, Podrez E. Platelet hyperreactivity, scavenger receptors and atherothrombosis. J Thromb Haemost 2009; 7(S1): 218-21.
  8. Stephen SL, Freestone K, Dunn S, Twigg MW, Homer-Vanniasinkam S, Walker JH, et al. Scavenger receptors and their potential as therapeutic targets in the treatment of cardiovascular disease. Int J Hypertens 2010; 2010: 646929.
  9. Imachi H, Murao K, Cao W, Tada S, Taminato T, Wong NC, et al. Expression of human scavenger receptor B1 on and in human platelets. Arterioscler Thromb Vasc Biol 2003; 23(5): 898-904.
  10. Fujisawa K, Tani P, McMillan R. Platelet associated. antibody to glycoprotein IIb/IIIa from chronic immune thrombocytopenic purpura patients often bonds to divalent cation dependent antigens. Blood 1993; 81: 1284-9.
  11. Hamidpour M, Behrendt M, Griffiths B, Par­tridge L, Lindsey N. The isolation and charac­terization of antiplatelet antibodies. Eur J Hae­matol 2006; 76: 331-8.
  12. Krieger M. Scavenger receptor class B type I is a multiligand HDL receptor that influences diverse
    physiologic systems. J Clin Invest 2001; 108(6): 793-7.
  13. Tsompanidi EM, Brinkmeier MS, Fotiadou EH, Giakoumi SM, Kypreos KE. HDL biogenesis and functions: role of HDL quality and quantity in atherosclerosis. Atherosclerosis 2010; 208(1): 3-9.
  14. Moore KJ, Freeman MW. Scavenger receptors in atherosclerosis beyond lipid uptake. Arterioscler Thromb Vasc Biol 2006; 26(8): 1702-11.
  15. Podrez EA, Byzova TV, Febbraio M, Salomon RG, Ma Y, Valiyaveettil M, et al. Platelet CD36 links hyperlipidemia, oxidant stress and a prothrombotic phenotype. Nat Med 2007; 13(9): 1086-95.
  16. Gawaz M. Platelets in the onset of atherosclerosis. Blood Cells Mol Dis 2006; 36(2): 206-10.
  17. Valiyaveettil M, Kar N, Ashraf MZ, Byzova TV, Febbraio M, Podrez EA. Oxidized high-density lipoprotein inhibits platelet activation and aggregation via scavenger receptor BI. Blood 2008; 111(4): 1962-71.
  18. Dole VS, Matuskova J, Vasile E, Yesilaltay A, Bergmeier W, Bernimoulin M, et al. Thrombocytopenia and platelet abnormalities in high-density lipoprotein receptor–deficient mice. Arterioscler Thromb Vasc Biol 2008; 28(6): 1111-6.
  19. Trigatti B, Rayburn H, Viñals M, Braun A, Miettinen H, Penman M, et al. Influence of the high density lipoprotein receptor SR-B1 on reproductive and cardiovascular pathophysiology. Proc Natl Acad Sci U S A 1999; 96(16): 9322-7.
  20. Huszar D, Varban ML, Rinninger F, Feeley R, Arai T, Fairchild-Huntress V, et al. Increased LDL Cholesterol and Atherosclerosis in LDL Receptor–Deficient Mice With Attenuated Expression of Scavenger Receptor B1. Arterioscler Thromb Vasc Biol 2000; 20(4): 1068-73.
  21. Ueda Y, Gong E, Royer L, Cooper PN, Francone OL, Rubin EM. Relationship between expression levels and atherogenesis in scavenger receptor class B, type I transgenics. J Biol Chem 2000;275(27):20368-73.
  22. Kozarsky KF, Donahee MH, Glick JM, Krieger M, Rader DJ. Gene transfer and hepatic overexpression of the HDL receptor SR-B1 reduces atherosclerosis in the cholesterol-fed LDL receptor–deficient mouse. Arterioscler Thromb Vasc Biol 2000; 20(3): 721-7.
 
 


 
 
 
 
Sci J Iran Blood Transfus Organ 2017; 14(2): 92-100
Original Article
 

 

The detection of Scavenger Receptor-B1 expression
and its role on the function of platelets in patients
with atherosclerotic disease
 
Abasalizade M.1, Nehzati P.1, Allahbakhshian M.1, Hamidpour M.1,
Khadem Mabodi A.A.1, Tabatabaie M.R.2
 
 
1School of Allied Medical Sciences, Shahid  Beheshti University of Medical Sciences, Tehran, Iran
2Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
 
 
 
Abstract
Background and Objectives
SR-B1 acts as the main receptor for HDL and plays an important role in reverse cholesterol transport. In contrast to LDL level which causes Atherosclerotic plaque in artery, plasma concentration of HDL correlates inversely with the incidence of coronary artery disease and atherosclerosis. This study investigates the expression of Scavenger Receptor-B1 and its role on the function of platelets in patients with atherosclerotic disease.
 
Materials and Methods
The present case-control study evaluated the SR-B1 receptor of 24 patients with atherosclerotic plaque and 24 healthy volunteers as the normal control. After separation of platelet, the expression of SR-BI was detected using western blotting. Platelet function in response to agonists was examined by aggregometry. Finally, the data were statistically analyzed using SPSS version 16 software and the student T-test.
 
Results
The western blotting assay showed a significant reduction of SR-BI protein expression in AD patients (0.4 AU) compared with the healthy control (1.4 AU), (p < 0.001). The platelet aggregation in response to ADP showed a significant increase in patients (85%) compared to the control (51%), (p < 0.001).
 
Conclusions 
The levels of SR-B1expression correlate inversely with platelet aggregation.
 
Key words: Platelets, Atherosclerosis, Patients
 
 
 
 
 
 
Received: 25 Oct 2016
Accepted:  1 Feb 2017
 
 

Correspondence: Hamidpour M., PhD of Hematology & Blood Banking. Associate Professor of Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences.
Postal code: 1971653383, Tehran, Iran. Tel: (+9821) 22717504; Fax: (+9821) 22717504
E-mail:
mohsenhp@sbmu.ac.ir
Type of Study: Research | Subject: Hematology
Add your comments about this article : Your username or Email:
CAPTCHA
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2025 CC BY-NC 4.0 | Journal of Iranian Blood Transfusion

Designed & Developed by: Yektaweb