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:: Volume 3, Issue 4 (Winter 2007) ::
Sci J Iran Blood Transfus Organ 2007, 3(4): 343-353 Back to browse issues page
Evaluation of cotransplantation of human mesenchymal stem cells and umbilical cord blood CD34+ cells with CFU-S assay
Bahman Delalat, Ali AKbar Pourfathollah , Ali Akbar Movassaghpour, Masoud Soleimani, Hosein Mazdarani, Saeed Kaviani, Soraya Rasi Ghaemi
Abstract:   (15180 Views)

 

  Abstract

 Background and Objectives

  Umbilical cord blood (UCB) contains a high number of primitive progenitor cells for transplantation. However, the rate of UCB CD34+ stem cell engraftment is low. In this study we examined the effect of human MSCs (mesenchymal stem cell) on engraftment of human UCB-derived CD34+ cells in irradiated Balb/c mice.

 

 Materials and Methods

  Human UCB CD34+ cells were obtained from full-term normal deliveries by immunomagnetic techniques and MSCs were isolated by a standard methodology from bone marrow aspirates. Isolated MSCs were evaluated for cell-surface antigens of CD166 and CD105 by flowcytometry. The absolute count of CD34+ UCB stem cells was also determined by flowcytometry. Irradiated (7 Gy) Balb/c mice were transplanted intravenously with 0.2 to 1.0 × 106 human UCB CD34+ cells in the presence or absence of 0.25 to 1 × 106 human bone marrow-derived MSCs. The mice in every group on day 11 after transplantation were killed and their spleen dissected. In every group, colony assay and H and E staining were performed. To make sure of the presence of human stem cells in spleen colonies, UCB CD34+ cells were labeled with super paramagnetic iron oxide (SPIO) before transplantation. The colonies in spleen then underwent Prussian blue staining. In the statistical analyis, Kruskal-Wallis test using SPSS software was employed to determine the number of colonies.

 

 Results

  Flowcytometry assay showed that after purification, 90% of the cells were CD34+ and 96% marker positive for MSCs. Viablity of cells was 100%. Cotransplantation of low doses of UCB CD34+ cells (0.2 and 0.3 × 106) and MSC (0.5 and 1 × 106) resulted in a significant increase of the number of colony forming units spleen, in comparison with the engraftment of UCB CD34+ stem cells without MSC after 11 days (p<0.01). Prussian blue staining showed the Fe+2 granules in UCB stem cells. This indicated that cells in the colonies were engrafted UCB CD34+ stem cells.

 

 Conclusions

  The results showed that cotransplantation of MSC with UCB CD34+ cells promotes engraftment of UCB CD34+ cells.

 

  Key words : Stem cell, Cotransplantation, Umbilical cord blood, Mesenchymal stem cell

Keywords: Stem cell, Cotransplantation, Umbilical cord blood, Mesenchymal stem cell
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Type of Study: Research | Subject: General
Published: 2014/08/18
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Delalat B, Pourfathollah A A, Movassaghpour A A, Soleimani M, Mazdarani H, Kaviani S et al . Evaluation of cotransplantation of human mesenchymal stem cells and umbilical cord blood CD34+ cells with CFU-S assay. Sci J Iran Blood Transfus Organ. 2007; 3 (4) :343-353
URL: http://bloodjournal.ir/article-1-123-en.html


Volume 3, Issue 4 (Winter 2007) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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