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:: Volume 10, Issue 3 (Autumn 2013) ::
Sci J Iran Blood Transfus Organ 2013, 10(3): 256-266 Back to browse issues page
Enhancement of the resistance of mesenchymal stem cells against killing conditions by the oxidative preconditioning
H. Bashiri Nahanji , M. Habibi Roudkenar , R. Halabian , M. Jalili , M.A. Jalili
Keywords: Hydrogen Peroxide (H2O2), Ischemic Preconditioning, Apoptosis, Multipotent Mesenchymal Stromal Cells
Full-Text [PDF 523 kb]   (2066 Downloads)     |   Abstract (HTML)  (12172 Views)
Type of Study: Research | Subject: Stem cells
Published: 2013/10/7
Full-Text:   (1535 Views)
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Original Article
 
 
 
 
Sci J Iran Blood Transfus Organ 2013; 10(3): 255-266
 
 
 
 

Enhancement of the resistance of mesenchymal
stem cells against killing conditions by the oxidative preconditioning
 
Bashiri Nahanji H.1, Habibi Roudkenar M.1, Halabian R.2, Jalili M.3, Jalili M.A.1
 
 
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
2Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
3Islamic Azad University, Karaj Branch, Karaj, Iran
 
 
Abstract
Background and Objectives
About 70% of MSCs die in the early stages of transplantation into the infarcted myocardium. Several solutions have been made to address this problem. In the last decade, preconditioning of MSCs with oxidative stresses has gained a lot of attention. In this study, we have investigated the effects of preconditioning with hydrogen peroxide (H2O2) on the survival of MSCs and their resistance against oxidative stresses.
 
Materials and Methods
Mesenchymal stem cells from bone marrow have been cultured. Cells from the passage four were treated with 5, 10, 15, 20, 30, 40, 50, 60, 80, and 100µm concentrations of H2O2, and were then recovered with the fresh medium. Finally, the treated cells were exposed to 500 µM H2O2 as the killing condition. The percentage of survived cells was analyzed by the MTT assay kit.
 
Results
Preconditioning with 5 and 10 µM H2O2 significantly increased the resistance of MSCs against the apoptosis induced by 500 µM H2O2.
 
Conclusions 
Preconditioning of MSCs with oxidative stresses enhances their survival; therefore, it can increase the efficacy of transplantation.
 
Key words: Hydrogen Peroxide (H2O2), Ischemic Preconditioning, Apoptosis, Multipotent Mesenchymal Stromal Cells
 
 
 
 
 
Received:  9  Sep 2012
Accepted:11 Feb 2013
 
 

Correspondence: Jalili MA., Phd of Medicinal Chemistry. Assistant Professor of Blood Transfusion Research  Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 82052155; Fax: (+9821) 88601599
E-mail: m.jalili@ibto.ir
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Bashiri Nahanji H, Habibi Roudkenar M, Halabian R, Jalili M, Jalili M. Enhancement of the resistance of mesenchymal stem cells against killing conditions by the oxidative preconditioning . Sci J Iran Blood Transfus Organ 2013; 10 (3) :256-266
URL: http://bloodjournal.ir/article-1-795-en.html
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Volume 10, Issue 3 (Autumn 2013) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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