Abstract

 Background and Objectives

 About 70% of MSCs die in the early stages of transplantation into the infarcted myocardium. Several solutions have been made to address this problem. In the last decade, preconditioning of MSCs with oxidative stresses has gained a lot of attention. In this study, we have investigated the effects of preconditioning with hydrogen peroxide (H₂O₂) on the survival of MSCs and their resistance against oxidative stresses .

 Materials and Methods

 Mesenchymal stem cells from bone marrow have been cultured. Cells from the passage four were treated with 5, 10, 15, 20, 30, 40, 50, 60, 80, and 100µm concentrations of H₂O₂, and were then recovered with the fresh medium. Finally, the treated cells were exposed to 500 µM H₂O₂ as the killing condition. The percentage of survived cells was analyzed by the MTT assay kit .

 Results

 Preconditioning with 5 and 10 µ M H₂O₂ significantly increased the resistance of MSCs against the apoptosis induced by 500 µ M H₂O₂ .

 Conclusions

 Preconditioning of MSCs with oxidative stresses enhances their survival therefore, it can increase the efficacy of transplantation .