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:: Volume 6, Issue 1 (Spring 2009) ::
Sci J Iran Blood Transfus Organ 2009, 6(1): 13-20 Back to browse issues page
Leukoreduction in packed cells filtered by home-made
H Abolghasemi Dr. , M Aghaiipour Dr., M Nikougoftar, N Amirizadeh Dr., M.T Mohammadi Dr., S Rahmani Dr., F Atashrazm, S Hadjati, P Zarei
Abstract:   (12172 Views)
Leukoreduction in packed cells filtered by home-made bedside filters prior and after optimization Abolghasemi H.1,2(MD), Aghaiipour M.1(MD), Nikougoftar M.1(MS), Amirizade N.1(PhD), Mohammadi M.T.3( MD),Rahmani S.4(MD), Atashrazm F.3(MS), Hadjati S.3(MS), Zarei P1.(BS) 1Iranian Blood Transfusion Organization, Research Center, Tehran, Iran 2Baghiatollah University of Medical Sciences, Tehran, Iran 3Iran University of Medical Sciences, Tehran, Iran 4Helal Iran Medical Devices Company, Karaj, Iran Abstract Background and Objectives Existence of leukocytes in all blood products causes a wide variety of side effects after transfusion. As a consequence, the use of filter technology for leukoreduction has been widely practiced. In this study, absolute leukocyte count in three types of home-made bedside filtered packed cell units is evaluated. Materials and Methods Ninety three packed cell units from blood donors were prepared in Tehran Regional Educational Blood Transfusion Center, stored at 4ºC, and filtered by two types of home-made filters within 1 hour at room temperature. The first type of filters was made prior to 2007 and the second type underwent optimization after 2007. Eight samples were filtered by control group filters with CE certificate. The results were analyzed with SPSS 11.5 and chi-square test. Results The mean values of leukocyte count/unit by CD45 and True Count Method were respectively 9×106 and 10×106 in 55 bags filtered by the first type filters and these values were 4.2×106 and 4.8×106 in 30 bags filtered by the second type filters, whereas the mean value of leukocyte count/bag in 8 bags filtered by control filters was 2.3×106. Conclusions After optimization of product technology and raw materials, the average number of leukocytes fell within the standard range. Twenty percent of cases contained more than 5×106 leukocytes and 80% less than that (CI95%= 5.7-34.3). Thus, following optimization, the differences in mean rates and SDs in both group two and control filters showed significant reduction and were measured to be within AABB standards.
Keywords: Leukocytes, Flow Cytometry, Iran
Full-Text [PDF 220 kb]   (1595 Downloads)    
Type of Study: Research | Subject: Hematology
Published: 2014/08/16
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Abolghasemi H, Aghaiipour M, Nikougoftar M, Amirizadeh N, Mohammadi M, Rahmani S, et al . Leukoreduction in packed cells filtered by home-made . Sci J Iran Blood Transfus Organ. 2009; 6 (1) :13-20
URL: http://bloodjournal.ir/article-1-298-en.html


Volume 6, Issue 1 (Spring 2009) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
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