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Leukoreduction in packed cells filtered by home-made
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H Abolghasemi   , M Aghaiipour , M Nikougoftar , N Amirizadeh , M.T Mohammadi , S Rahmani , F Atashrazm , S Hadjati , P Zarei |
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Abstract: (15733 Views) |
| Leukoreduction in packed cells filtered by home-made
bedside filters prior and after optimization
Abolghasemi H.1,2(MD), Aghaiipour M.1(MD), Nikougoftar M.1(MS), Amirizade N.1(PhD),
Mohammadi M.T.3( MD),Rahmani S.4(MD), Atashrazm F.3(MS), Hadjati S.3(MS), Zarei P1.(BS)
1Iranian Blood Transfusion Organization, Research Center, Tehran, Iran
2Baghiatollah University of Medical Sciences, Tehran, Iran
3Iran University of Medical Sciences, Tehran, Iran
4Helal Iran Medical Devices Company, Karaj, Iran
Abstract
Background and Objectives
Existence of leukocytes in all blood products causes a wide variety of side effects after transfusion. As a consequence, the use of filter technology for leukoreduction has been widely practiced. In this study, absolute leukocyte count in three types of home-made bedside filtered packed cell units is evaluated.
Materials and Methods
Ninety three packed cell units from blood donors were prepared in Tehran Regional Educational Blood Transfusion Center, stored at 4ºC, and filtered by two types of home-made filters within 1 hour at room temperature. The first type of filters was made prior to 2007 and the second type underwent optimization after 2007. Eight samples were filtered by control group filters with CE certificate. The results were analyzed with SPSS 11.5 and chi-square test.
Results
The mean values of leukocyte count/unit by CD45 and True Count Method were respectively 9×106 and 10×106 in 55 bags filtered by the first type filters and these values were 4.2×106 and 4.8×106 in 30 bags filtered by the second type filters, whereas the mean value of leukocyte count/bag in 8 bags filtered by control filters was 2.3×106.
Conclusions
After optimization of product technology and raw materials, the average number of leukocytes fell within the standard range. Twenty percent of cases contained more than 5×106 leukocytes and 80% less than that (CI95%= 5.7-34.3). Thus, following optimization, the differences in mean rates and SDs in both group two and control filters showed significant reduction and were measured to be within AABB standards. |
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| Keywords: Leukocytes, Flow Cytometry, Iran |
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Full-Text [PDF 220 kb]
(4129 Downloads)
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Type of Study: Research |
Subject:
Hematology
Published: 2014/08/16
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