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:: Volume 20, Issue 2 (Summer 2023) ::
Sci J Iran Blood Transfus Organ 2023, 20(2): 90-100 Back to browse issues page
The effect of gamma-irradiation on platelet metabolic state inPRP-platelet concentrates during storage
F. Kiani nodeh , M. Ghasemzadeh , ٍE.H. Hosseini
Keywords: Key words: Gamma Rays, Platelet-Rich Plasma, Graft vs Host Disease
Full-Text [PDF 570 kb]   (267 Downloads)     |   Abstract (HTML)  (475 Views)
Type of Study: Research | Subject: Blood Transfusion
Published: 2023/07/1
Full-Text:   (219 Views)
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Sci J Iran Blood Transfus Organ 2023;20 (2): 90-100
Orginal Article
 





The effect of gamma-irradiation on platelet metabolic state in PRP-platelet concentrates during storage

Kiani Nodeh F.1, Ghasemzadeh M.1, Hosseini E.1



1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran


Abstract
Background and Objectives
Gamma irradiation (GI) of platelet concentrates (PCs) is done to prevent the risk of Transfusion associated graft versus host disease (TA-GVHD). However, some of studies indicate a lower post-transfusion responses and aggravation of platelet storage lesion (PSL) after GI. In this study, the effect of GI on the platelet metabolic status in stored PRP-PCs was investigated.

Materials and Methods
in our experimental study, 10 PRP-PC were randomly obtained from Iranian Blood Transfusion Organization (IBTO). Then, two ABO- and D matched PRP-PC units were pooled and splited into two bags, one kept as control while other was gamma-irradiated with 30 Gray. Within 7-day storage, glucose concentration and LDH activity were measured by enzymo-colorimetric method. PLT count and pH were measured using a hematology whole blood analyzer and the pH meter, respectively. Swirling was evaluated by visual assessment. Statistical data were analyzed by Kruskal-Wallis test with Dunn’s multiple comparison test and Wilcoxon Mached-Pair test. P values of less than 0.05 were considered to be significant.

Results
Swirling was maintained in both groups throughout the storage period. Platelet count significantly decreased on Days 5 and 7 of storage but there was no significant difference between irradiated and non-irradiated PCs. Glucose decreased whilst LDH activity increased during storage period. The increments of LDH activities were significant on Days 5 (P<.05) and 7 of storage (P<.01) in non-irradiated groups.

Conclusions 
The quality of irradiated PCs is well maintained throughout the storage period, with minimal difference in metabolic parameters compared to non-irradiated PLTs.

Key words: Gamma Rays, Platelet-Rich Plasma, Graft vs Host Disease




Received:  8 Feb 2023
Accepted: 1 Mar 2023



Correspondence: Hosseini E., PhD in Hematology. Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 88699531; Fax: (+9821) 88699531
E-mail:
1- Acridine Orange
1- Biological safety cabinet
1- Platelet Concentrate
2- Food and Drug Administration
3- Normal Skin Flora
4- Platelet Rich Plasma-Platelet Concentrate
5- Eosin-Methylene blue
6- Thioglycolate
1- Acridine Orange
1- Biological safety cabinet
1- Platelet Concentrate
2- Food and Drug Administration
3- Normal Skin Flora
4- Platelet Rich Plasma-Platelet Concentrate
5- Eosin-Methylene blue
6- Thioglycolate
e.hosseini10@yahoo.com.au

 
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Kiani nodeh F, Ghasemzadeh M, Hosseini ٍ. The effect of gamma-irradiation on platelet metabolic state inPRP-platelet concentrates during storage. Sci J Iran Blood Transfus Organ 2023; 20 (2) :90-100
URL: http://bloodjournal.ir/article-1-1487-en.html
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Volume 20, Issue 2 (Summer 2023) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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