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The effect of gamma-irradiation on platelet metabolic state inPRP-platelet concentrates during storage
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F. Kiani nodeh   , M. Ghasemzadeh , ٍE.H. Hosseini |
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Abstract: (467 Views) |
Abstract
Background and Objectives
Gamma irradiation (GI) of platelet concentrates (PCs) is done to prevent the risk of Transfusion associated graft versus host disease (TA-GVHD). However, some of studies indicate a lower post-transfusion responses and aggravation of platelet storage lesion (PSL) after GI. In this study, the effect of GI on the platelet metabolic status in stored PRP-PCs was investigated.
Materials and Methods
in our experimental study, 10 PRP-PC were randomly obtained from Iranian Blood Transfusion Organization (IBTO). Then, two ABO- and D matched PRP-PC units were pooled and splited into two bags, one kept as control while other was gamma-irradiated with 30 Gray. Within 7-day storage, glucose concentration and LDH activity were measured by enzymo-colorimetric method. PLT count and pH were measured using a hematology whole blood analyzer and the pH meter, respectively. Swirling was evaluated by visual assessment. Statistical data were analyzed by Kruskal-Wallis test with Dunn’s multiple comparison test and Wilcoxon Mached-Pair test. P values of less than 0.05 were considered to be significant.
Results
Swirling was maintained in both groups throughout the storage period. Platelet count significantly decreased on Days 5 and 7 of storage but there was no significant difference between irradiated and non-irradiated PCs. Glucose decreased whilst LDH activity increased during storage period. The increments of LDH activities were significant on Days 5 (P<.05) and 7 of storage (P<.01) in non-irradiated groups.
Conclusions
The quality of irradiated PCs is well maintained throughout the storage period, with minimal difference in metabolic parameters compared to non-irradiated PLTs.
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| Keywords: Key words: Gamma Rays, Platelet-Rich Plasma, Graft vs Host Disease |
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Full-Text [PDF 570 kb]
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Type of Study: Research |
Subject:
Blood Transfusion
Published: 2023/07/1
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