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:: Volume 14, Issue 2 (Summer 2017) ::
Sci J Iran Blood Transfus Organ 2017, 14(2): 84-91 Back to browse issues page
The allele frequencies of human Neutrophil Antigens 5 (HNA-5) in Tabriz city
Atieh Khosravi , Karim Shams Asanja Dr., Mojgan Shaiegan Dr. * , Shahram Samiei , Zahra Ataee , Maryam Abdollahi , Mahnaz Kavari , Mahboobeh Shafiei almasian , Parisa Hosseinzadeh
Abstract:   (1834 Views)


Background and Objectives

Human neutrophil antigens include 5 antigen systems. These antigens are on the plasma membrane of white blood cells. The aim of the study is to know about HNA allele frequency in the population in order to estimate the prevalence of the diseases related to HNA-5.

Materials and Methods

In this descriptive study, blood samples were taken from 190 Azari unrelated blood donors having referred to Tabriz Blood Center. DNA extraction was performed using commercial kit Pars Tous. HNA-5a and HNA-5b molecular were then evaluated by PCR-RFLP. PCR products were digested using enzymes Sdu1 Bsp1286I.  HNA-5a+ and HNA-5a- (or HNA-5b) antigen gene frequencies were calculated using the Hardy-Weinberg equation.


HNA-5a (+/+), HNA-5a (+/-) and HNA-5a (-/-) phenotypes were observed in 93, 10 and 87 patients, respectively. The observed frequencies were consistent with Hardy-Weinberg equilibrium; allele frequencies of HNA-5a+ and HNA-5a- (or HNA-5b) were equal to 0.51 and 0.49, respectively.


HNA-5 alleles frequency rates were in the same range reported for the other populations.

Keywords: Key words: Neutrophils, PCR, Blood Donors
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Type of Study: Research | Subject: Imunology
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khosravi A, Shams Asanja K, Shaiegan M, Samiei S, Ataee Z, Abdollahi M, et al . The allele frequencies of human Neutrophil Antigens 5 (HNA-5) in Tabriz city. Sci J Iran Blood Transfus Organ. 2017; 14 (2) :84-91
URL: http://bloodjournal.ir/article-1-1106-en.html

Volume 14, Issue 2 (Summer 2017) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
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