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Evaluation of the repair effects of MSCs cultivated with secretome of Nrf2 engineered MSCs on acute kidney injury in rats
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F. Jaleh   , F. Amiri , M. Dehghan Harati , M. Habibi Roudkenar , M.A. Jalili |
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Abstract: (5248 Views) |
Abstract
Background and Objectives
The decrease in mesenchymal stem cells (MSCs) survival rate after transplantation is a major challenge in MSC-cell-therapy. Hence, it is promising to employ some proper strategies for addressing this problem. This study was conducted to assay the therapeutic effects of MSCs treated with Nrf2-manipulated-MSC conditioned medium in acute kidney injury (AKI)-induced animal models.
Materials and Methods
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In an experimental study, recombinant plasmid pcDNA3.1-Nrf2 was transfected into bone marrow-derived MSCs and the resulted conditioned medium was harvested. The MSCs was cultivated with Nrf2-manipulated-derived conditioned medium. The conditioned medium-treated-MSCs were transplanted to AKI-induced rats (each group containing 10 rats) and the therapeutic potentialities of these MSCs were evaluated using biochemical and pathological methods.
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Results
Fourteen days after MSCs transplantation, there was a significant difference in BUN decreasing in rat groups injected with conditioned medium-treated-MSCs (48 ± 3.5 mg/dL) in comparison with those injected with normal MSCs (100 ± 9.9 mg/dL) (p < 0.001). The number of observed casts (0.26) in kidney tissue sections of these rat groups were also less than (0.51) the groups transplanted with normal MSCS.
Conclusions
Cultivation of MSCs in the presence of Nrf2-manipulated-derived conditioned medium increase the therapeutic effects of these cells in AKI-induced models.
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| Keywords: Key words: Mesenchymal Stem Cells, Acute Kidney Injury, Conditioned Medium |
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Full-Text [PDF 654 kb]
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Type of Study: Research |
Subject:
Stem cells
Published: 2016/12/24
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