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:: Volume 13, Issue 1 (Spring 2016) ::
Sci J Iran Blood Transfus Organ 2016, 13(1): 1-10 Back to browse issues page
Induction of cell death and apoptosis in NB4 promyelocytic leukemic cells using Oligonucleotide as a telomerase antagonist
L. Asghari kia , D. Bashash , S.H. Ghaffari , M. Hamid poor , A. Ghavamzadeh
Abstract:   (6447 Views)

Abstract

Background and Objectives

Acute promyelocytic leukemia (APL) is characterized by a specific t(15;17), distinct morphologic picture, and a clinical coagulopathy that contributes to the morbidity and mortality of the disease. Telomerase is consistently activated in nearly all APL patients and telomerase-mediated telomere stabilization is responsible for unlimited replicative potential of the disease. This study aimed to investigate the effects of designed oligonucleotide as a telomerase inhibitor on NB4 cells.

Materials and Methods

NB4 leukemic cells were treated with various concentrations of oligonucleotide by transfection method using lipofectamin 2000. The inhibitory effect of oligonucleotide on cell metabolic activity and cell viability was assessed by MTT assay. Quantitative real-time PCR were applied to investigate alteration of Bax and Bcl-2 mRNA levels.

Results

Oligonucleotide decreased cell viability index and exerted cytotoxic effect against NB4 leukemic cells; we found that exposing cells with Oligonucleotide at 40 pMol for 48 h induced cell death and apoptotic effects on NB4 cells. Furthermore, transcriptional suppression of Bcl-2 and upregulation of Bax were found upon NB4 treatment by oligonucleotide.

Conclusions

Based on the short telomere length and high terlomerase activity in APL as well as inhibitory effect of oligonucleotide against NB4 cells, anti-telomerase-based therapy might be regarded as a successful strategy in APL therapy.

Keywords: Key words: Acute Promyelocytic Leukemia, Apoptosis, Cell Death
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Type of Study: Research | Subject: Hematology
Published: 2016/03/12
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Asghari kia L, Bashash D, Ghaffari S, Hamid poor M, Ghavamzadeh A. Induction of cell death and apoptosis in NB4 promyelocytic leukemic cells using Oligonucleotide as a telomerase antagonist . Sci J Iran Blood Transfus Organ 2016; 13 (1) :1-10
URL: http://bloodjournal.ir/article-1-949-en.html


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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 13, Issue 1 (Spring 2016) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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