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:: Volume 9, Issue 4 (Winter 2013) ::
Sci J Iran Blood Transfus Organ 2013, 9(4): 363-371 Back to browse issues page
Arsenic trioxide-induced apoptosis in HL-60 cell line by decreasing the ratio of Bcl-2 to Bax mRNA expression
SH. Rostami , S. Abroun , K. Alimoghaddam , M. Norouz Nia , B. Chahardouli , A. Ghavamzadeh
Abstract:   (17672 Views)

  Abstract

 Background and Objectives

 Arsenic trioxide ( As2O3 ) has been shown to have anti-cancer effects on a wide range of cancers. As2O3 has proved to be the most efficient in treatment of acute promyelocytic leukemia (APL) patients. It primarily acts by triggering apoptosis in cancer cells. To better understand the molecular mechanisms involved in As2O3 -induced apoptosis, in the present study we examined the effects of As2O3 both on the expression of Bax and Bcl-2 (the major regulators of apoptosis), and p53 genes in HL-60 cell line.

 

 Materials and Methods

 In this experimental study, the MTT colorimetric assay was used to assess the growth-inhibitory effect of As2O3 on HL-60 cells. The mRNA levels of p53, Bax and Bcl-2 were analyzed by using Real-time PCR. Data were analyzed by using SPSS18, t-test and ANOVA tests .

 

 Results

  As2O3 inhibits the growth of HL-60 cells in a dose dependent manner at 24h . Significant decrease (89% ± 3%) in cell viability was observed at 4μM and the IC50 value was about 16 μM. In HL-60 cell, no expression of p53 was detected. Real-time PCR results showed while Bax mRNA expression was not significantly affected in As2O3 treated HL-60, the Bcl-2/Bax ratio expression level significantly decreased in a dose dependent manner.

 

 Conclusions

  As2O3 exerts part of its antitumor effect by inducing apoptosis through decreasing Bcl-2/Bax mRNA ratio in p53-null leukemic cell line HL-60. More research on the protein level is required for these results to be reinforced.

 

Keywords: Key words: arsenic trioxide, Bax Protein, Genes, bcl-2
Full-Text [PDF 406 kb]   (2817 Downloads)    
Type of Study: Research | Subject: Hematology and Oncology
Published: 2013/08/27
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Rostami S, Abroun S, Alimoghaddam K, Norouz Nia M, Chahardouli B, Ghavamzadeh A. Arsenic trioxide-induced apoptosis in HL-60 cell line by decreasing the ratio of Bcl-2 to Bax mRNA expression. Sci J Iran Blood Transfus Organ 2013; 9 (4) :363-371
URL: http://bloodjournal.ir/article-1-711-en.html


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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 9, Issue 4 (Winter 2013) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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