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:: Volume 7, Issue 4 (Winter 2011) ::
Sci J Iran Blood Transfus Organ 2011, 7(4): 214-220 Back to browse issues page
Detection of the Lepore and Asian-Indian beta-globin gene deletions with duplex-PCR
F. Rahmi Nezhad , R. Ali Mohammadi , P. Fuladi , S. Foroughi , M. Feiz Pour , R. Vahidi , M. Hashemi , F. Mola Zadeh , M. Heidari , M. Deilam Salehi , S. Zeinali
Abstract:   (15582 Views)
Abstract Background and Objectives Beta-thalassemia is one of the most common single-mutation diseases. These mutations cause decreased beta-globin protein synthesis or even deletion. Most of the mutations in beta-globin gene are point mutations but there are some small and big deletions in beta-globin gene cluster. The goal of this research is to detect and study two deletions (Lepore, Asian-Indian) with duplex-PCR. Materials and Methods This study was done on 3 Asian-Indian deletion samples and 10 Lepore samples. After detecting two deletions (Lepore and Asian-Indian), we started setting Gap-PCR in these two known deletions. Duplex-PCR with 8 different primers for detecting Lepore and Asian-Indian deletions simultaneously was set at 68ºC annealing temperature these two deletions can be detected easily in one reaction with this new method. Results The results show that detecting Lepore and Asian-Indian deletions with multiplex PCR is practical, and the specific bands for each deletion are detectable. Conclusions Detecting these two deletions in one reaction (Duplex-PCR) is practical. This method is easy, reliable, and fast it is also economical and saves both time and money. Key words: Beta-thalassemia, Beta-globins, Gene deletion
Keywords: Beta-thalassemia, Beta-globins, Gene deletion
Full-Text [PDF 241 kb]   (3130 Downloads)    
Type of Study: Research | Subject: Genetis
Published: 2014/07/26
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Rahmi Nezhad F, Ali Mohammadi R, Fuladi P, Foroughi S, Feiz Pour M, Vahidi R, et al . Detection of the Lepore and Asian-Indian beta-globin gene deletions with duplex-PCR. Sci J Iran Blood Transfus Organ 2011; 7 (4) :214-220
URL: http://bloodjournal.ir/article-1-436-en.html


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Volume 7, Issue 4 (Winter 2011) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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