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:: Volume 2, Issue 5 (Autumn 2005) ::
Sci J Iran Blood Transfus Organ 2005, 2(5): 171-181 Back to browse issues page
Relative sensitivity of third generation hepatitis C virus antibody detection assays: evaluation of 20 kits
S. Amini Kafi-Abad * , A. Talebian , M. Maghsudlu , S. Raman
Abstract:   (25262 Views)

  Abstract

  

 Background and Objectives

  The most important challenge in selecting suitable assays for the detection of anti-HCV is sensitivity. In this study, 20 assays (EIA method) were compared with each other and with anti-HCV 3.0 Enhanced SAV (Ortho Company production) as the reference assay recommended by WHO .

 

  Materials and Methods

  20 kits were compared by 3 to 4 seroconversion and 2 to 3 performance panels. The relative sensitivity of kits was calculated based on WHO recommendations.

 

 Results

  In seroconversion panels, relative sensitivity of 3 assays was the same as the reference assay and 5 assays showed lower relative sensitivity, but the differences between these five kits and the reference assay appeared just in two samples. In performance panels, two assays came out to be the same as the reference assay and the other 5 assays detected just 2 samples to have a level lower than anti-HCV 3. In all seroconversion and performance panels, the best results were obtained by ETI-AB-HCH-K4 (146) (Diasorin), Monalisa Anti-HCV plus Version 2 (BIO-RAD), Hepanostica Anti-HCV ULTRA (BIOMERIEUX), Anti-HCV-EIA 3rd (Avicenna Medial Center), and HCV AB (DIA PRO).

 

 Conclusions

  For improvement of blood safety, the assay with high sensitivity is recommended to be used, and the samples with weak positive reactions especially in seroconversion and low titer performance panels should be given more attention.

  

  

 Key words: Antibody , Hepatitis C, Elisa, Sensitivity, Seroconversion panel

Keywords: Antibody, Hepatitis C, Elisa, Sensitivity, Seroconversion panel
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Type of Study: Research | Subject: General
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Amini Kafi-Abad S, Talebian A, Maghsudlu M, Raman S. Relative sensitivity of third generation hepatitis C virus antibody detection assays: evaluation of 20 kits. Sci J Iran Blood Transfus Organ. 2005; 2 (5) :171-181
URL: http://bloodjournal.ir/article-1-42-en.html


Volume 2, Issue 5 (Autumn 2005) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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