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:: Volume 6, Issue 3 (Autumn 2009) ::
Sci J Iran Blood Transfus Organ 2009, 6(3): 199-207 Back to browse issues page
Detection and assessment of the frequency of NPM1 and FLT3 ITD mutations in acute myeloid leukemia patients
V. Pazhakh , F. Zaker , K. Ali Moghadam , F. Atashrazm
Abstract:   (14552 Views)

  Abstract

 Background and Objectives

 NPM1/Nucleophismin/B23 is a phosphoprotein with high expression in the proliferating cells. NPM1+AMLs are also frequently associated with FLT3 ITD mutations. The purposes of this study were to assess the frequency of NPM1 and FLT3 ITD mutations among Iranian AML patients and their correlation with FAB subtypes of AML.

 

 Materials and Methods

 Bone marrow and peripheral blood samples of 131 AML patients were randomly collected, and their DNA was then extracted. Afterwards, PCR was applied to the fragment of NPM1 gene with specific primers. PCR products were electrophoresed using CSGE method. In the end, the positive samples were sequenced to confirm the presence of NPM1 mutations. Furthermore, FLT3 ITD positive cases were screened using PCR and 2.5% agarose gel electrophoresis.

 

 Results

 Out of 131 patients, 23 (17.55%) (CI 95% = 0.107-0.244) were known to have NPM1 gene mutations. The highest frequency was among the subtypes of M4 (30.4%), M3 (21.7%), and M5 (13%). Also, 21 samples (16.03%) (CI 95% = 0.092-0.229) had FLT3 ITD mutations with 8 cases being NPM1 positive and other 13 NPM1 negative.

 Conclusions

 NPM1 mutations are more frequent in monocytic subtypes (M4, M5). High frequency rates of NPM1 in M3 subtypes and allele D mutations in all subtypes together with the high degree of association between occurrence of NPM1 and FLT3 ITD mutations could be considered as interesting findings of the study (p=0.012).

 

 Key words : Leukemia, Myeloid, Acute, PCR, Electrophoresis, Agar Gel

 

Keywords: Leukemia, Myeloid, Acute, PCR, Electrophoresis, Agar Gel
Full-Text [PDF 323 kb]   (4501 Downloads)    
Type of Study: Research | Subject: Hematology and Oncology
Published: 2014/08/17
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Pazhakh V, Zaker F, Ali Moghadam K, Atashrazm F. Detection and assessment of the frequency of NPM1 and FLT3 ITD mutations in acute myeloid leukemia patients. Sci J Iran Blood Transfus Organ 2009; 6 (3) :199-207
URL: http://bloodjournal.ir/article-1-348-en.html


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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 6, Issue 3 (Autumn 2009) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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