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:: Volume 2, Issue 3 (Spring 2005) ::
Sci J Iran Blood Transfus Organ 2005, 2(3): 13-21 Back to browse issues page
Detection of hepatitis B virus DNA (PCR) in HBsAg negative, anti-HBc positive blood donors in Tehran province
S. Amini Kafi-abad * , A. Talebian , F. Ranjbar Kermani , M. Moghtadaie , M. Sobhani , Sh. Samie
Abstract:   (29769 Views)



 Background and Objectives

  Screening the blood donors for serological markers reduced the incidence of transfusion-transmitted infections especially post-transfusion hepatitis C. However, there remains residual risk due to pre-seroconversion period. HCV RNA (PCR) of blood donations reduced the residual risk of transfusion-transmitted HCV infection. In this study, blood donations were screened for HCV RNA by RT-PCR method.


  Materials and Methods 

  An extra plasma sample was collected from 1026 blood donors. 1000 out of 1026 samples were negative for HBsAg, anti-HCV (EIA, third generation), anti-HIV and RPR. Every 5 samples were pooled. The sensitivity of HCV-RNA detection by RT-PCR method was 380 geq/ml according to Proficiency VQC panel. 1000 donations in 200 pools were tested.



 False reactivity of samples considered positive accounts for 5.5% of cases, and 5.5% were invalid due to non-specilic bands. 6% of the pools were false-positive. A false positive result was defined as positive on initial testing but negative on repeat single testing. However, all of the samples were negative for HCV RNA by RT-PCR method.



 No sample was found to be serologically negative and HCV RNA positive. However, further studies are recommended for further clarification.



Key words : Blood donation, Donor screening, Hepatitis C virus, plasma, Pooled, PCR
Keywords: PCR, Blood donors, HBs Ag
Full-Text [PDF 443 kb]   (2173 Downloads)    
Type of Study: Research | Subject: General
Published: 2014/06/30
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Amini Kafi-abad S, Talebian A, Ranjbar Kermani F, Moghtadaie M, Sobhani M, Samie S. Detection of hepatitis B virus DNA (PCR) in HBsAg negative, anti-HBc positive blood donors in Tehran province. Sci J Iran Blood Transfus Organ. 2005; 2 (3) :13-21
URL: http://bloodjournal.ir/article-1-21-en.html

Volume 2, Issue 3 (Spring 2005) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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