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:: Volume 4, Issue 4 (winter 2008) ::
Sci J Iran Blood Transfus Organ 2008, 4(4): 285-295 Back to browse issues page
Malignant B cell lymphomas vs benign lymphoproliferations and the role of immunoglobulin heavy chain gene rearrangement analysis
B. Poopak , Z. Latifzadeh , H. Abolghasemi , A. Yousefian , G. Khosravipoor , K. Farahani , M. Jahangirpour
Abstract:   (14374 Views)

  Abstract

 Background and Objectives

 Malignant lymphoma may be very difficult to be diagnosed through routine histopathological methods because they may mimic reactive architecture or contain reactive infiltrates. Detection of the monoclonal lymphocyte population is one of the best methods of diagnosis since a monoclonal proliferation is strongly suggestive of neoplasia. By means of a PCR method it is possible to detect the immunoglobulin heavy chain (IgH) gene rearrangement and consequently the lymphocyte clonality.

 

 Materials and Methods

 We evaluated formalin-fixed, paraffin-embedded biopsies, and bone marrow aspiration of 12 cases with non definite or suspicious histopathological diagnosis of non Hodgkin B cell lymphoma. After DNA extraction and quality control, semi-nested PCR was performed using consensus primers for amplification of CDR-3 region. PCR products were analyzed after heteroduplex analysis using polyacrylamide gel electrophoresis and silver staining.

 

 Results

 75% and 16.6% of patients showed clonal and polyclonal patterns respectively. One case showed weak monoclonal band in smear background and remained suspicious after duplicate PCR.

 

 Conclusions

 Our findings are comparable with other international studies and support the concept that molecular techniques such as PCR provide a helpful approach in detection of monoclonal immunoglobulin rearrangements in malignant lymphoma. This is especially true for suspicious cases, but always in combination with clinical and histopathological information.

  

 Key words : B-cell Lymphoma, Immunoglobulin Heavy Chain, Gene rearrangement 

 

Full-Text [PDF 266 kb]   (3549 Downloads)    
Type of Study: Research | Subject: Hematology and Oncology
Published: 2014/08/17
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Poopak B, Latifzadeh Z, Abolghasemi H, Yousefian A, Khosravipoor G, Farahani K et al . Malignant B cell lymphomas vs benign lymphoproliferations and the role of immunoglobulin heavy chain gene rearrangement analysis. Sci J Iran Blood Transfus Organ 2008; 4 (4) :285-295
URL: http://bloodjournal.ir/article-1-194-en.html


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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 4, Issue 4 (winter 2008) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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