[Home ] [Archive]   [ فارسی ]  
:: Main :: About us :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 1, Issue 1 (Autumn 2004) ::
Sci J Iran Blood Transfus Organ 2004, 1(1): 1-9 Back to browse issues page
Flow cytometric survey of RBC chimerism after bone marrow transplantation
M. Shaiegan * , S. Hadjati , M. Iravani , M. Aghaipour , G. David , D. Bernard+ , A.S. Tabatabaeeian , M.H. Lotfi , P. Lotfi
Abstract:   (28203 Views)

 

  Abstract

 

 Background and Objectives

  The aim of the present study was to evaluate red blood cell chimerism after bone marrow transplantation by flow cytometry.

 

 Materials and Methods

  In order to perform this assay, FITC labeled antibodies against blood groups ABH, Rh, Kell, Duffy, Kidd, MNS were used.14 hematologic patients under BMT were selected for this study. The required sample was 5 ml peripheral blood that is collected in tubes containing EDTA. At first, donor and recipients red cells phenotypes were identified with the use of both agglutination and flow cytometry methods then, on post-transplantation days of 15, 30 and 60, only blood samples of the recipients were analyzed by flow cytometry for the antigens differing from donors to recipients. Antibody screening test and titration of ABH Isohemagglutinins were performed on recipients' plasma samples and then repeated on post- transplantation day of 60.

 

 Results

  After BMT, red cell chimerism was detected in all 14 patients (in 9 patients on post-transplantation day of 15 and in 5 patients on day of 30). Antibodies against minor blood groups and Rh blood group were not detected at all. The occurrence of chimerism was not inhibited by ABO incompatibility of donors and recipients but in patients who were ABH incompatible with their donors, ABH isohemagglutinins titer following transplantation decreased. Although the presence of isohemagglutinins did not prevent chimerism but it seems these antibodies by attaching to their related antigens on chimeric red cells membrane prevented corresponding antigen detection.

 

 Conclusions

  Now by using flow cytometry, red cell phenotyping is applicable and reticulocyte analysis is much easier to perform so that chimerism can be detected in patients who have recently experienced blood transfusion. Moreover, through further evaluation of red cell chimerism and detection of recipient autologous red cells, disease relapse can be predicted.

 

 

 Key words: Bone Marrow Transplantation (BMT), Chimerism, Red cells, Blood group antigens, Hematological disorders

 

Keywords: Bone Marrow Transplantation (BMT), Chimerism, Red cells, Blood group antigens, Hematological disorders
Full-Text [PDF 404 kb]   (2702 Downloads)    
Type of Study: Research | Subject: General
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Shaiegan M, Hadjati S, Iravani M, Aghaipour M, David G, Bernard+ D, et al . Flow cytometric survey of RBC chimerism after bone marrow transplantation. Sci J Iran Blood Transfus Organ. 2004; 1 (1) :1-9
URL: http://bloodjournal.ir/article-1-1-en.html


Volume 1, Issue 1 (Autumn 2004) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
Persian site map - English site map - Created in 0.06 seconds with 32 queries by YEKTAWEB 3781