Scientific Journal of

  Abstract

 Background and Objectives

 White blood cells (WBCs) in blood products are the major stimulus for a number of detrirmental biological reactions including febrile nonhemolytic transfusion reactions, alloimmunization against HLA antigens, and cytomegalovirus transmission. In this study, our objective was to study the effect of storage time on the filtration of platelet concentrates (PCs). The total number of white blood cells as well as the distribution of WBC subsets in PC units filtered before and after storage was compared.

 Materials and Methods

 In this experimental study, platelet-rich plasma-derived PCs (5 pooled units) were filtered both fresh and after 5 days of storage then, the total number of WBCs was calculated by flow cytometry and cell counting. WBC subsets were analyzed by flow cytometry with three-color fluorescence. In this study, the third genaration bags and filters were used.

 Results

 Before filtration, the total number of WBC was significantly higher in fresh units compared with stored units, whereas in postfiltration samples the number of white blood cells was significantly lower in the fresh compared with the stored units. Although the absolute number of WBCs significantly reduced, filtration induced significant changes in proportion in subsets in both fresh and stored units the percentage of B cells and monocytes increased after filtration.

 Conclusions

 Both pre- and post-storage WBC filtration affect the proportions of WBCs in the final product but pre-storage WBC filtration of platelet concentrates is superior than post-storage WBC filtration.

 Key words : White blood cells, Platelet concentrates, Blood, Filtration 

  Abstract

 Background and Objectives

 Hematological parameters are influenced by physical activities. The purpose of this study was to survey the response of hematological factors to a circuit resistance training program with different intensities in athlete male students. 

 Materials and Methods

 In this experimental study, 24 athlete male students (age, 24.46 ± 0.46 years weight, 75.58 ± 1 . 29 kg height, 177 . 25 ± 1.32 cm) took part in this study voluntarily. Subjects were randomly divided into three groups including three low, moderate and high intensities. Each group contained 8 people. Circuit resistance training program included ten stations, which took place in three sets with twenty seconds for each station. Subjects were asked to fast for 12 hours. Then, 10 ml blood samples pre and post exercise were taken from arm vena. All data were analyzed by using descriptive and inferential statistics (paired-samples t and ANOVA) through SPSS 16 software.

 Results

 Post exercise results showed a significant increase in the values of Hct and RBC in low intensity group, in Hct, RBC, Hb, and MCV in moderate intensity group, and in MCV, Hb, Hct, RBC, and WBC in high intensity group (p< 0.05) . Also, significant decrease was seen in the values of MCHC and PV in moderate intensity group and PV, MCHC, and MCH in high intensity group (p< 0.05).

 Conclusions

 Results showed circuit resistance training with progressively low, moderate and high intensities could specifically influence hematologic factors. Hence, it is recommended to coaches to consider intensity in designing circuit resistance training programs for athletes.

  Abstract

 Background and Objectives

 The aim of this quasi-experimental study is to investigate the effect of 6 weeks of progressive endurance training and Silymarin supplementation intake on hematological parameters in sedentary men (age: 25 ± 5 years, weight 86 ± 21, height 178 ± 13 , BMI 27 ± 5).

 Materials and Methods

 To this end, twenty-one students from Shahrood University of Technology who were willing to participate in the study were chosen for the study. The subjects were then randomly divided into supplemental groups (n=11) and placebo groups (n=10). Both groups received progressive endurance training. The control group received placebo while the experimental or supplemental group received Silymarin. Blood samples were collected before and after 6 weeks of exercise training and supplementation intake. Hematological parameters such as RBC, MON, RBC, HCT, PLT were measured. Data were then analyzed using repeated measure (2×2).

 Results

 Hematological parameters such as RBC, MON, RBC, HCT, PLT were measured. Data were analyzed using repeated measure (2×2).

 Conclusions

 The results of this study showed that Silymarin supplementation and progressive endurance training not only increases hemoglobin factor but also reduces monocytes, erythrocytes, and hematocrit factors significantly (P≤0.05).

  Abstract

 Background and Objectives

 The accumulation of white particulate matter in blood bags, with the mechanism of formation unknown, has been rarely reported. However, the high number of platelets, blood lipids, and the like could help the white matter form.

 Case

 A 450 mL blood bag coated with 63 mL CPDA1 including the white matter detected was transferred from the blood center to the central quality control department. All test results on RBCs, white cells, platelets, hemoglobin, hematocrite, protein sera, triglyceride, and cholestrol were negative. The donor had no record of any medicine consumption. The microbial culture of white matter was negative.

 Conclusions

 Although no correlation has been clearly found between the accumulation of white matter and transfusion reactions, it is recommended to prevent administering the white matter including blood bags to the patient these blood bags should be quarantined for further analysis. The appearance of the blood bags before transfusion should be checked.