Atieh Khosravi, Dr. Karim Shams Asanja, Dr. Mojgan Shaiegan, Shahram Samiei, Zahra Ataee, Maryam Abdollahi, Mahnaz Kavari, Mahboobeh Shafiei Almasian, Parisa Hosseinzadeh,
Volume 14, Issue 2 (5-2017)
Abstract
Abstract
Background and Objectives
Human neutrophil antigens include 5 antigen systems. These antigens are on the plasma membrane of white blood cells. The aim of the study is to know about HNA allele frequency in the population in order to estimate the prevalence of the diseases related to HNA-5.
Materials and Methods
In this descriptive study, blood samples were taken from 190 Azari unrelated blood donors having referred to Tabriz Blood Center. DNA extraction was performed using commercial kit Pars Tous. HNA-5a and HNA-5b molecular were then evaluated by PCR-RFLP. PCR products were digested using enzymes Sdu1 Bsp1286I. HNA-5a+ and HNA-5a- (or HNA-5b) antigen gene frequencies were calculated using the Hardy-Weinberg equation.
Results
HNA-5a (+/+), HNA-5a (+/-) and HNA-5a (-/-) phenotypes were observed in 93, 10 and 87 patients, respectively. The observed frequencies were consistent with Hardy-Weinberg equilibrium; allele frequencies of HNA-5a+ and HNA-5a- (or HNA-5b) were equal to 0.51 and 0.49, respectively.
Conclusions
HNA-5 alleles frequency rates were in the same range reported for the other populations.
B. Rahmati, Dr. M. Zadsar, Sh. Samiee, Dr. M. Shaigan,
Volume 19, Issue 3 (10-2022)
Abstract
Abstract
Background and Objectives
HNAs are glycoprotein antigens on the surface of neutrophils. Antibodies against these antigens are produced in patients with repeated blood transfusions. We here study the
HNA-1a, HNA-1b and HNA-1c gene frequencies in regulary transfused patients suffering from hemolytic transfusion reactions as well as the risk of alloimmunization of these individuals based on the genotype pattern.
Materials and Methods
As the study population, 99 thalassemia patients were included in the descriptive study. Sampling lasted from July 2019 to March 2020 at Zafar Thalassemia Center. Patients were administered low-leukocyte blood. Three mL of blood was collected in tubes containing EDTA and DNA was extracted. HNA-1 gene was analyzed by PCR-SSP method. Allelic frequency of neutrophil antigens was calculated using Hardy-Weinberg equation. Chi-square and T-test were used to analyze the data.
Results
The frequency rates of observed alleles were 52.7% for HNA-1a, 44.3% for HNA-1b, 3% for HNA-1c, which was in accordance with Hardy-Weinberg's law with a 5% confidence interval. The highest risk of alloimmunization to HNA-1b, HNA-1a, and HNA-1c were 22%, 18%, and 3%, respectively.
Conclusions
The frequency of HNA-1 neutrophil antigen allele in thalassemia patients with febrile blood transfusion was consistent with previous findings in different Iranian populations. Patients still have a risk of alloimmunization and blood transfusion based on neutrophilic genotype pattern.
