Abstract

Background and Objectives

Neutrophil alloantigens are involved in several blood transfusion reactions including transfusion related acute lung injury (TRALI), refractoriness to granulocyte transfusions and febrile transfusion reactions (FNHTRs). Due to lack of reports on neutrophil antigens,  we aimed to detect the frequency rates of HNA-3a and HNA-3 b antigens in some blood donors living in Tehran.

Materials and Methods

Blood samples were collected in EDTA (anticoagulant) containing tubes from 110 blood donors having referred to Tehran Blood Center. DNA was extracted by silica filtered columns. PCR-SSP was used for evaluation of HNA-3a, HNA-3b. The gene frequency rates of HNA 3a and HNA-3b antigenes were calculated by  Hardy Weinberg equilibrium.

Results

The gene frequencies of HNA-3a, HNA-3b were 0.74 and 0.26, respectively showing consistency with the Hardy-Weinberg equilibrium. HNA-3a phenotypic frequency was found in 103 (93.6%) blood donors while 7 (6.4%) showed lacking it. HNA-3b was found in 50 (45.5%) blood donors while 60 (54.5%) lacking. There was not found any differences in gene and antigene frequency rates between the two genders.

Conclusions

The frequencies of HNA-3a and -3b were similar to those found in the previous study conducted on German and Turkish populations.