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Showing 3 results for Hla-Drb1

F. Yari, N. Bagheri, M. Zaman Vaziri, M. Sobhani, F. Sabaghi, A. Talebian,
Volume 4, Issue 3 (9-2007)
Abstract

 

  Abstract

 Background and Objectives

 Major Histocompatibility Complex (MHC) is the most polymorphic system in the genome of different species. In human beings, these genes named HLA are located on the chromosome 6. HLA class I and II undertake genetic control of the immune system. Identification of HLA alleles is useful in transplantation, disease, and anthropological studies. Among different antigens of HLA, DR (DRB1) antigens are the most variable. In this research, due to the importance of DRB1 antigens in bone marrow transplantation, these antigens were studied in normal population. This study was performed on different Iranian races and did not suffice to a specific group.

 

 Materials and Methods

 DNA was extracted from the whole blood sample of 466 normal individuals after randomized sampling. Some HLA-DRB locus segments were amplified using 23 primer pairs by using PCR-SSP method. Finaly, PCR products were evaluated by electrophoresis in 2% agarose gel.

 

 Results

 The most prevalent alleles in DRB1 locus in normal poulation of Iran were DRB1*11, DRB1*13, DRB1*15, and DRB1*04 (20%, 11.4%, 11.4%, 10%, respectively). Whereas DRB1* 09 was the least frequent allele.

 

 Conclusions

 This research showed genetic diversity of HLA DRB1 in the mixed Iranian population. The data suggest that the Iranian population share certain HLA class II genetic components with the populations residing in Russia and Eastern and Southern European countries.

 

 Key words : HLA-DRB1, PCR, Iran

 


Dr. M. Shaiegan, Dr. H. Abolghasemi, Dr. F. Yari, Dr. M. Paridar, Dr. M. Maghsudlu, Dr. S. Amini Kafiabad, Dr. Sh. Kashani, Dr, A, Heydari, Dr. A. Gharehbaghian, F. Sabaghi, Dr. M. Sobhani, M. Amani, M Taheri, M. Paknejad,
Volume 10, Issue 3 (9-2013)
Abstract

  Abstract

 Background and Objectives

 The distribution of HLA alleles varies among different ethnic populations. Obtaining HLA data for different ethnic groups will be helpful to determine donor recruitment goals and strategies in unrelated stem cell registries.

  

 Materials and Methods

 Based on the data available from the Iranian Stem Cell Donor Registry, the frequency rates of HLA-A, B, DRB1 alleles evaluated by PCR-SSP method were reported 1513 individuals living in Tehran city with six different ethnicities (Fars, Azeri, Kurd, Lur, Gilak, and Mazani) were the participants.

  

 Results

 Out of 1513 participants, Fars and Azeri ethnic groups had the highest number with 63.12% and 20.02%, respectively. Twenty one HLA-A, thirty-one HLA-B, and thirteen HLA-DRB1 alleles were observed. Data analysis among different ethnicities showed no significant differences between Fars and Azeries except for HLA-DRB1*33 frequency (p< 0.005). Significant differences between Fars and Kurds were seen in HLA-A*03/11 and HLA-B*08/51 frequencies. There were significant differences between Fars and Gilaks in HLA-A*03/26, HLA-B*38/52 frequencies (p< 0.05).

  

 Conclusions

 The number of reported alleles in this study was similar to previous ones. There is not much alleles diversity, despite a few differences, across the different ethnic groups.

 

 Key words: HLA-A, HLA-B, HLA DRB1, Allele Frequency, Iran

 


Dr. F. Yari, M. Zaman Vaziri, N. Bagheri, Dr. A. Teimourpour, F. Sabaghi, F. Mortezapour Barfi,
Volume 21, Issue 2 (6-2024)
Abstract

Abstract
Background and Objectives
This study looked at the frequency of HLA alleles and their genetic diversity in specific Iranian ethnic groups. This is important for the successful transplantation of hematopoietic stem cells.

Materials and Methods
In this descriptive study, HLA-DRB1 alleles were determined in low resolution level for donors using the PCR-SSP method. By using the HLA lab data and the stem cell donors’ information in Iranian Blood Transfusion Organization, the ethnic and HLA information from the Gilak (n = 510, 24.70%), Lur (n = 465, 22.53%), Kurd (n = 719, 34.84%), and Arab (n = 370, 17.93%) ethnicities were collected and analyzed. The relationship between alleles and ethnicity was examined, and a statistically significant relationship (p < 0.05) between the frequency of alleles in the studied ethnic groups was evaluated using the chi-square test.

Results
The low frequency allelic groups related to HLA-DRB1 were HLA-DRB1*09 (0.6%), HLA-DRB1*12 (0.8%), and HLA-DRB1*08 (1.6%). Allelic groups with higher frequency included HLA-DRB1*11 (22.7%), HLA-DRB1*03 (11.8%), HLA-DRB1*15 (11.7%), and HLA-DRB1*04 (11.1%). Among the allelic groups of HLA-DRB1, the frequency of 9 HLA-DRB1 allelic groups showed significant differences in the studied populations.

Conclusions 
The frequency of several allelic groups showed significant differences among Iranian ethnicities. Identifying similarities and differences in the frequency of HLA alleles among Iranian ethnicities can help not only expand the pool of donors in registries but also assist in planning for providing of hematopoietic stem cell donors for patients from different Iranian ethnic groups.

 


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