Scientific Journal of

  Abstract

 Background and Objectives

  Although the French-American-British (FAB) Classification system is the basis for the diagnosis and treatment of AML, it has its own limitations. During recent years immunophenotyping by flow cytometry is widely used for the identification of AML'S subtypes. Immunophenotyping has been especially helpful in discrimination of AML with monocytic differentiation (M4-M5) from nonmonocytic subtypes (M0- M1- M2- M3- M6- M7). However several studies have indicated that CD14 mostly is negative when applied to leukemias with monocyte differentiation.

  Materials and Methods 

  Some studies have shown that CD64 (FC g RI) is an early and specific myelo-monoid marker. In this study we evaluated CD14 and CD64 antibodies to identify cells of monocytic lineage in 216 cases of AML who were referred to IBTO flow cytometry laboratory. These monoclonal antibodies prepared by DAKO company and were conjugated with Phyco Erythrin. The samples were analysed by Epics-x1 Flow cytometer. The markers were considered positive if 20% or more of the cells expressed it. The Chi-square test was also used in SPSS software.

 Results

  Results revealed that CD64 was highty specific (88%) and sensitive (67%) and CD14 was highty specific (96%) but not sensitive (31%) with >95% confidence rate. These results are compatible with other studies.

 Conclusions

  Finally because of high sensitivity of CD64, it should be considered in all of immunophenotyping protocols as a sensitive and specific marker for monoid cells.

  Abstract

 Background and Objectives

 The process of platelet concentrate production by plasma rich (PRP) method could activate the platelet and granules secretion of beta thromboglobulin, LDH and CD62P. Platelets activated during the preparation process do not have sufficient efficiency for hemostasis in vivo. It seems that platelet preparation by buffy coat method has an ability less than PRP to activate the platelet. Measuring platelet activation indices, such as CD62P expression and beta thromboglobulin, is a useful means to evaluate the percentage of activated platelet concentrates and compare the two methods of buffy coat and PRP.

 Materials and Methods

 In this experimental study, 15 concentrates were prepared via PRP method and 15 via BC method 15 intact blood units were also considered as control group. The percentages of CD62P expression, soluble CD62P concentrates, IL-8 level, and CD14 positive cells were evaluated. Special monocolonal antibodies that conjugated with flourecence dye in flocytometric method were used for CD62P and CD14. ELISA method was used for evaluation of soluble CD62P and IL-8.

 Results

 The average platelet count in both methods showed no significant difference, but WBC contamination rate in PRP-PCs was more than BC-PCs. In PRP-PCs, we found a little decrease in CD62P expression and increase in soluble form and IL-8 level during reservation time. The level of CD14 showed no significant difference in these components. In BC method during the three day reservation, expression of CD62P, its soluble form, and IL-8 concentrates increased and the level of monocyte surface CD14 showed slight decrease ranging from 0.4 to 0.1.

 Conclusions

 It is concluded that there is a close relationship between IL-8 and WBC count in platelet concentrates. In PRP method in contrary to BC method, high speed centrifuge causes adhesion, aggregation and platelet activation.

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 Key words : Platelet, Platelet rich plasma, CD62P, IL-8, CD14.�