Search published articles


Showing 6 results for Paz
Detection and assessment of the frequency of NPM1 and FLT3 ITD mutations in acute myeloid leukemia patients
V. Pazhakh, Dr. F. Zaker, Dr. K. Ali Moghadam, F. Atashrazm,
Volume 6, Issue 3 (Autumn 2009)
Abstract

  Abstract

 Background and Objectives

 NPM1/Nucleophismin/B23 is a phosphoprotein with high expression in the proliferating cells. NPM1+AMLs are also frequently associated with FLT3 ITD mutations. The purposes of this study were to assess the frequency of NPM1 and FLT3 ITD mutations among Iranian AML patients and their correlation with FAB subtypes of AML.

 

 Materials and Methods

 Bone marrow and peripheral blood samples of 131 AML patients were randomly collected, and their DNA was then extracted. Afterwards, PCR was applied to the fragment of NPM1 gene with specific primers. PCR products were electrophoresed using CSGE method. In the end, the positive samples were sequenced to confirm the presence of NPM1 mutations. Furthermore, FLT3 ITD positive cases were screened using PCR and 2.5% agarose gel electrophoresis.

 

 Results

 Out of 131 patients, 23 (17.55%) (CI 95% = 0.107-0.244) were known to have NPM1 gene mutations. The highest frequency was among the subtypes of M4 (30.4%), M3 (21.7%), and M5 (13%). Also, 21 samples (16.03%) (CI 95% = 0.092-0.229) had FLT3 ITD mutations with 8 cases being NPM1 positive and other 13 NPM1 negative.

 Conclusions

 NPM1 mutations are more frequent in monocytic subtypes (M4, M5). High frequency rates of NPM1 in M3 subtypes and allele D mutations in all subtypes together with the high degree of association between occurrence of NPM1 and FLT3 ITD mutations could be considered as interesting findings of the study (p=0.012).

 �

 Key words : Leukemia, Myeloid, Acute, PCR, Electrophoresis, Agar Gel

 


Methylenetetrahydrofolate reductase polymorphisms and susceptibility to childhood ALL
F. Atashrazm, Dr. F. Zaker, Dr. M. Aghaeipour, Dr. V. Pazhakh,
Volume 6, Issue 4 (Winter 2010)
Abstract

  Abstract

 Background and Objectives

 Genetic variations and mutations are of the etiological factors leading to leukemia. Among these genetic alterations, polymorphisms are present on gene surfaces of some critical molecules. The aim of this study was to assess individual and/or combined role of these two polymorphisms (C677T and A1298C) in resistance against pediatric acute lymphoblastic leukemia. Moreover, the frequency rate of these important polymorphisms has not been reported in Iran so far and the present study is the first attempt to this end.

 

 Materials and Methods

 Using PCR and RFLP analyses, we studied the prevalence rate of the C677T and A1298C Methylenetetrahydrofolate (MTHFR) genotypes in 103 pediatric ALL patients and 160 age-sex matched control patients. The data were analyzed with Hardy-weinberg and Chi-square by SPSS 16.

 �

 Results

 No significant association between two common polymorphisms of MTHFR or combination of polymorphisms with the risk of ALL was observed. The study also showed the high prevalence of A1298C which was significantly higher than that reported for most Asian population (40.67% in our study versus 17-19% in Asian). It is proved that C677T prevalence pattern is similar with those in most Asian populations .

 

 Conclusion

 Our findings suggest that the MTHFR C677T and A1298C gene variants do not have a major influence on the susceptibility to pediatric ALL. But despite the absence of any significant association, the frequency of MTHFR 677TT was lower among patients than general population which may support previous evidence about its protective effect against ALL.

 

  Key words : Polymorphism(Genetic), Methylene Tetrahydrofolate Reductase, Lymphoblastic Leukemia, Acute


Investigation of anti-HBc and anti-HBs prevalence in HBsAg negative blood donors in Sistan-o-Baluchestan province
M. Pazoukian, Dr. Z. Sharifi, Dr. A.a Pourfatholah, Dr. M. Hamidpour, Dr. E. Sanei Moghaddam, Dr. S. Khosravi,
Volume 10, Issue 3 (Autumn 2013)
Abstract

  Abstract

 Background and Objectives

 The most common marker used for HBV infection diagnosis in blood donors is screening for HBsAg. The failure of HBsAg detection in blood donors cause of either the window period, or the mutation in S gene, or the reduction in the antigen synthesis can lead to transfusion-transmitted HBV in blood recipients. There have been reported hepatitis cases following the transfusion of anti-HBc positive blood and blood components. The prevalence of anti-HBc in populations is proportional to the prevalence of HBsAg. It is better then for studies about the prevalence of anti-HBc to be conducted in HBV highly prevalent regions. Thus, the aim of this study was to survey anti-HBc and anti-HBs prevalence rates in blood donors in Sistan-o-Baluchestan.

  

 Materials and Methods

 In this cross-sectional study, 1500 HBsAg negative samples were collected from Sistan-o-Baluchestan and tested for anti-HBc. All anti-HBc positive samples were tested for anti-HBs. All data were analyzed statistically using Chi-square test.

  

 Results

 Out of 1500 blood donors who were negative for HBsAg, 144 (9.6%) were positive for anti-HBc with 74.3% having the immunity titer of anti-HBs.

 

 Conclusions

 In this study, the prevalence rate of anti-HBc was very high. Most of anti-HBc positive blood donors were positive for anti-HBs. Therefore, screening of blood donors for anti-HBc can limit the storage of blood.

 


Effect of curcumin and sorafenib on AKT gene expression in KG1 and U937 cell lines
R. Pazahr, M. Mohammadikian, Dr. E. Ali Asgari, Dr. S. Mohammadi, Dr. Sh. Rostami, Dr. B. Chahardolii, Dr. D. Babakhani, Dr. M. Nikbakht,
Volume 17, Issue 2 (Summer 2020)
Abstract
Abstract
Background and Objectives
Acute myeloid leukemia is a heterozygous hematologic malignancy that is manifested by the     accumulation of hematopoietics stem cells in peripheral blood and bone marrow. Anticancer effects and  cryotoxic activity of curcumin have been proven frequently in many cancers. Sorafenib is known as an inhibitor of angiogenesis which prevent cells’ survival. In the present study, the effects of curcumin and sorafenib and their combination were evaluated on AML cells.
 
Materials and Methods
In this experimental study, U937 and KG-1 cells were treated with different concentration of curcumin and sorafenib and their combination.  MTT assay was applied to assess the viability of cells.  Percentage of apoptotic cells was evaluated by annexin PI staining. Also Real Time PCR was performed to investigte the level of  AKT mRNA expression.
 
Results
Our data showed that the percentage of apoptotic cells significantly increased in response to treatment with  curcumin (40µM in KG-1 and U937 cell lines), sorafenib (5 µM and 7 µM in U937 and KG-1, respectively) and their combination. Moreover, the mRNA level of AKT gene was downregulated in KG-1 and U937 cells.
 
Conclusions 
Our results suggest that combination of curcumin and sorafenib could lead to promote apoptosis. Furthurermore, downregulation of AKT gene shows that these agents can be considered as effective agents on AML cells. 
 

Investigating natural mutations in BCP and preC regions of hepatitis B virus in blood donors with chronic hepatitis B anti-HBe+
Dr. Z. Sharifi, A. Yadegari, Z. Paz,
Volume 21, Issue 1 (Spring 2024)
Abstract
Abstract
Background and Objectives
Various mutations can occur in the HBV genome during chronic HBV infection due to error-prone viral replication and stress on the host immune system. This study aimed to determine the mutations in the BCP and preC regions of the hepatitis B virus genome in asymptomatic carriers and its role in the persistence of hepatitis B infection.

Materials and Methods
In this descriptive study conducted by easy non-probability sampling method in 2017, 40 asymptomatic blood donors positive in terms of HBsAg and confirmatory tests and negative in terms of anti-HCV and anti-HIV tests were included in the study. Anti-HBc, HBe Ag and anti-HBe (Total) tests and ALT and AST tests were performed. Nested-PCR reaction was performed on the extracted DNA and the sequences were determined. Data analysis was done using online software https://hbv.geno2pheno.org.

Results
Out of the 40 examined samples (94.8%), 38 were men and 2 (5.2%) women with an average age of 38.6 ± 8.6. Mutations in (G1764A) and BCP (A1762T) were observed in 10 (50%) and 8 (40%) cases, respectively, and G54T17 mutation was observed in 3 (15%) samples out of 20 HBV positive and HBeAg negative samples. The mutation in the preC region and the nucleotide position A1896 G was found in 12 cases (60%).

Conclusions 
The highest frequency of mutations was observed in G1764A and BCP which can be considered as one of the effective factors in not clearing and removing the virus by the host's immune system and causing chronic hepatitis B infection.

 

The Level of Expression of Programmed Death Type1 During the Natural Course of Hepatitis B Virus Infection in Asymptomatic Carriers of Hepatitis B
Dr. Zohreh Sharifi, Abbas Yadgari, Zahra Paz,
Volume 21, Issue 4 (Winter 2024)
Abstract
A B S T R A C T
Background and Objectives
In chronic hepatitis B virus (HBV) infection, the balance between viral replication and the host immune response plays a critical role in liver disease progression. Acquired immune responses, particularly cellular immune responses, are believed to lead to HBV clearance. Programmed cell death type 1(PD-1) negatively regulates T cell activation, proliferation, and cytokine production, contributing to chronic HBV infection by inhibiting the function of virus-specific CD8+ T cells. In this study, we examined the expression level of PD-1 in asymptomatic donors with hepatitis B during the natural course of HBV infection.
Materials and Methods
This case-control study was conducted on 120 blood donors who were divided into two groups. The control group consisted of 60 healthy individuals who tested negative for HBsAg, while the case group comprised 60 asymptomatic HBV-infected blood donors who tested positive for HBsAg. HBsAg-positive samples were tested for anti-HBc total, HBeAg, and anti-HBe, along with alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels. To analyze gene expression, RNA was extracted, and complementary DNA (cDNA) was synthesized. Real-Time PCR was performed to measure the expression of the PD-1 gene as well as the β-actin reference gene. Changes in gene expression were analyzed using REST software, with a significance threshold of p< 0.05.
Results
All asymptomatic donors with hepatitis B tested positive for total anti-HBc and anti-HBe, while HBeAg was not detected. The ALT and AST enzyme levels were measured at 29 ± 0.11 IU/L and 28 ± 0.21 IU/L, respectively. Gene expression analysis was conducted using REST software, based on cycle thresholds obtained from Real-Time PCR for both the PD-1 gene and the reference gene. A 1.5-fold increase in PD-1 gene expression was observed in asymptomatic HBV-infected donors compared to healthy individuals; however, this difference was not statistically significant.
Conclusions 
The expression of the PD-1 gene remains stable during the natural course of chronic HBV infection in asymptomatic individuals and shows no significant difference compared to healthy individuals.
 

Page 1 from 1     

© 2025 CC BY-NC 4.0 | Scientific Journal of Iran Blood Transfus Organ

Designed & Developed by : Yektaweb