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Evaluation of the effects of MIP1- on ex vitro expansion of hematopoietic progenitor cells in different culture media for the purpose of cord blood transplantation
K Alimoghaddam, M Khalili, M Soleimani, P Ghodsi, P Hayat, M Mohyedin, L Moezi, A Arjmand, A Ghavamzadeh,
Volume 3, Issue 2 (Summer 2006)
Abstract

  Abstract

 Background and Objectives

 Umbilical cord blood (CB) has been identified both as a rich source for hematopoietic stem cells (HSCs) and as an alternative to bone marrow transplantation. To accelerate the pace of engraftment in cord blood (CB) transplantation, HSCs should be ex vivo expanded. So the aim of this study was to evaluate the effects of macrophage inflammatory protein-1 alpha (MIP1- a ) on ex vivo expansion of HSCs and its role in prevention of HSCs differentiation in different conditions.

 

 Materials and Methods

 Mononuclear cells (MNCs) were seperated from cord blood and cultured in RPMI 1640 with 10% fetal calf serum (FCS) or 10% cord blood plasma (CBP) or serum free media (SF). Culture media contained 50 ng/ml of interlukin 6 (IL-6), interlukin 3 (IL-3),


 thrombopoietin (TPO), stem cell factor (SCF), and flt3-ligand. MNCs were cultured for 2 weeks in the presence or absence of MIP1- a in CO2 incubator expansion potential was then studied by MNCs counting and flowcytometry for CD34+/CD38- cells in 7 and 14 days.

 

 Results

 In all MIP1- a contained media, more increase in expansion of CD34+/CD38- and CD34+ cells was observed.

 

 Conclusions

 These results suggest that the effect of MIP1- a depends on type of medium and the cytokines present in the culture it also shows that addition of MIP1- a is useful for clinically ex vivo expansion of cord blood-derived progenitor cells.

  

 Key words : MIP1- a , Cord blood, Ex vivo expansion, Hematopoietic progenitor cells 


Pattern of blood component utilization in Arak, Iran
Dr. F.s. Mahdaviani, Dr. I. Ahmadi Iraqi, Dr. M. Khalili, L. Khosrowpour, H. ٍemyaei, A.r. Moradabadi, Sh. Abbasi, M. Khalili,
Volume 16, Issue 4 (Winter 2019)
Abstract
Abstract
Background and Objectives
Blood transfusion in medical care is one of the main treatments. However, blood transfusion is not without risk. Investigating the patterns of blood products utilization can help improve blood products usage and predict future blood products demand. Therefore, in this study, the pattern of blood product utilization in Arak hospitals has been investigated.
 
Materials and Methods
In this descriptive cross-sectional study, four hospitals in Arak were selected and the desired information including type and amount of utilized blood products and thier relationship with patient characteristics such as age, gender, diagnosis of the disease were evaluated during the first 6 months of 1397 (according to Iranian Calendar). Diseases were classified based on the International Classification of Diseases, 10th. Edition (ICD-10).
 
Results
All 7300 patients, 54.4% of whom male, received 6928 RBC units, 3829 FFP units, 8196 random platelet units and 171 apheresis platelet units in all four hospitals. In all three types of utilized products (RBC, FFP, platelet), the recipient group with the age range of 41-64 years received the highest rate of blood products and the highest utilization of RBC, FFP, platelet  pertained to patients with neoplasm. The average utilization of RBC was 1.9 ± 2.05 units, FFP was 2.7 ± 2.35 units and platelet was 3.7±4.05 units, and the most utilized product in all four hospitals was RBC.
 
Conclusions 
The results of the current study represents the blood components utilization by age, sex and various diseases in Arak with regard to the relationship between the usage of blood components and the demographic pattern of the region. Therefore, the feasibility of using the obtained pattern in order to predict and plan for the blood components needed for this region based on the demographic pattern of Arak city will be possible.
 

 

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