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Showing 9 results for Heidari
Asvadi-Kermani I., Evazie Ziaeei J., Nikanfar A.r., Maljaie H., Golchin M., Berahmani G., Dolatkhah R., Mahmmoudpour A., Dehkhoda R., Moghaddam A., Torabi S., Hosseinpoor-Panahi A., Rajabzadeh A., Soleimani F., Heidari S., Ardabilzadeh Sh.,
Volume 2, Issue 6 (Winter 2006)
Abstract
Abstract Background and Objectives Cancer patients due to bone marrow suppression, sepsis, and other relevant complications require appropriate blood components for transfusion. However, there are risks of transfusion reactions. These reactions are influenced by many factors varying across different geographic regions and medical centers. This study was performed to determine incidence of early transfusion reactions and their clinical symptoms and signs in cancer patients, and to calculate the correlation of these adverse reactions with some demographic data and some specifications of blood components. Materials and Methods In this descriptive study, 39 reactions from 4023 blood transfusion attempts were assessed. Patients were monitored for symptoms and changes in vital signs within 24 hours following transfusion. Data analysis was performed by descriptive and inferential statistics (X2 and ANOVA). Results The majority of reactions belonged to platelets (56.43%) and packed cells (43.58%). The most common symptoms were shown to be rigors (2.72%), and fever (2.33%). The incidence rate was estimated to be 2.7% for FNHTR, and 2.1% for allergic reactions. Haptoglobin deficiency was found in 0.8% of FNHTR cases. A correlation was observed between sex and history of previous reactions (p=0.048, p=0.04, respectively) with blood transfusion reactions. Conclusions These findings indicated that incidence of blood transfusion reactions and clinical symptoms correlated with other studies. The incidence of reactions in women and individuals with previous history of repeated blood transfusions was shown to be greater. Transfusion of packed cell was associated with allergic reactions (p=0.04), and high platelet lifetime associated with pulmonary reactions (p=0.044). Key words: Blood transfusion, Transfusion reaction, Cancer patients
Dr. M. Zeinalian, Dr. A.r. Moafi, Dr. R. Fadaie Nobari, Dr. K. Heidari,
Volume 6, Issue 2 (Summer 2009)
Abstract
Abstract Background and Objectives Although major beta-thalassemia prevention and control program is one of the most successful programs in genetics field, there are new cases of major beta-thalassemia due to laboratory errors. So the laboratory quality control (QC) is very important to diagnose carriers in preventive programs. At present, there is not a distinct and measurable index for function assessment and comparison in screening laboratories this urged us to create an objective, measurable and explicit index for health care givers. For this purpose, we compared the function of screening labs in districts of Isfahan Province. Materials and Methods In this cross-sectional study, we collected the data from "pre-marriage screening program" in 18 districts (19 screening labs) covered by Isfahan University of Medical Sciences from 2006 to 2008. The percentage rates of "screened males with low blood indexes" in every 3 months (P index) were assessed then, the average and coefficient variants in duration of 3 years were calculated with excel software. Based on these findings, "screening labs’ functions" were assessed and compared. Results The least "CVs of P index" within 19 labs were found to be in Mobarakeh laboratory (10.4%) and Provincial reference lab (10.9%), respectively. The highest CVs were found in Khour (76.3%) and Tiran and Karvan labs (58.2%), respectively. The P index average in all centers was 14% (13.9% in reference lab) with the highest being in Mobarakeh(24.2%) and the lowest in Khour(8.8%). Conclusions Whereas the number of marriage candidates in each screening lab is relatively stable, the centers with the highest CV value of P index are probably lower in function level as compared with the centers with the lowest CV value of P index (as in reference screening lab). CV of P index may be a good evidence for quality of "screening lab function" and should be evaluated annually. The screening labs with high CV of P index need more attention and evaluation. � Key words : Thalassemia, screening, Iran�
F. Rahmi Nezhad, R. Ali Mohammadi, P. Fuladi, S. Foroughi, M. Feiz Pour, R. Vahidi, M. Hashemi, F. Mola Zadeh, M. Heidari, M. Deilam Salehi, Dr. S. Zeinali,
Volume 7, Issue 4 (Winter 2011)
Abstract
Abstract
Background and Objectives
Beta-thalassemia is one of the most common single-mutation diseases. These mutations cause decreased beta-globin protein synthesis or even deletion. Most of the mutations in beta-globin gene are point mutations but there are some small and big deletions in beta-globin gene cluster. The goal of this research is to detect and study two deletions (Lepore, Asian-Indian) with duplex-PCR.
Materials and Methods
This study was done on 3 Asian-Indian deletion samples and 10 Lepore samples. After detecting two deletions (Lepore and Asian-Indian), we started setting Gap-PCR in these two known deletions. Duplex-PCR with 8 different primers for detecting Lepore and Asian-Indian deletions simultaneously was set at 68ºC annealing temperature these two deletions can be detected easily in one reaction with this new method.
Results
The results show that detecting Lepore and Asian-Indian deletions with multiplex PCR is practical, and the specific bands for each deletion are detectable.
Conclusions
Detecting these two deletions in one reaction (Duplex-PCR) is practical. This method is easy, reliable, and fast it is also economical and saves both time and money.
Key words: Beta-thalassemia, Beta-globins, Gene deletion
Dr. B. Poopak, Dr. Sh. Taghdisi, Dr. M. Heidari, P. Fallah, G. Khosravipour, Sh. Bolouri, T. Golkar,
Volume 11, Issue 3 (Autumn 2014)
Abstract
Abstract Background and Objectives Clopidogrel is one of the most commonly prescribed drugs to prevent ischemic events following coronary syndromes or stent placement. The objective of this study was to evaluate the prevalence of the cytochrome P450 (CYP450) 2C19 enzyme (CYP2C19) genotypes in the Iranian population. Materials and Methods CYP2C19 (*1/*2/*3) variants were analyzed using Polymerase Chain Reaction-Restriction Length Polymorphism (PCR–RFLP) assays in a representative sample of 154 Iranian patients with ischemic heart disease in Peyvand Laboratory. Results In this study, out of 154 Iranian patients the frequency rates of CYP2C19 *2 were 36 (23.4%) in heterozygous and 6 (3.9%) in homozygous forms 112 (72.7%) patients had the normal genotype. Conclusions FDA recommendations are more useful to be practiced in our country than other countries. Physicians should identify patients and advise them to consider other antiplatelet medications or alternative dosing strategies in poor metabolizers.
M. Shirzad, Dr. E. Heidarian, Dr. B. Poorgheisari, N.a. Amini Sarteshnizi, Z. Soorani, P. Beshkar,
Volume 12, Issue 2 (Summer 2015)
Abstract
Abstract Background and Objectives Acute lymphoblastic leukemia (ALL) is a neoplastic disorder and a common malignancy in children. Apoptosis is a normal physiological process which occurs during the maintenance of tissue hemostasis. The defect in this process leads to the development of cancer. It can be induced by a variety of treatments, such as cytotoxic chemotherapy. Hesperetin, a flavonoid isolated from the red fruits, has been examined with regard to the inhibition of proliferation and induction of apoptosis in pancreatic cells. Hesperetin also activates NOTCH-1 and tumor suppressor in carcinoid tumor. Materials and Methods In this fundamental-applied research, the cell lines of C121 (Jurkat cell) were cultured in RPMI supplemented with 10% fetal bovine serum (FBS) and Penicilin/streptomycin. The growth and survival of cells with various concentrations of hesperetin was evaluated by MTS kit. The amount of cell death by trypan blue staining and flow cytometry as well as by apoptosis kit (Partec) was examined. Results Lymphoblastic cell lines (C121) in exposure to different concentrations of hesperetin were affected and ΜM 200 drug concentration at 48 hours was reported as inhibitory concentration or IC50. The mode of cell death induced by hesperetin was found to be apoptosis, as judged by the morphologic alteration of the cells and by Anexin v conjugated with FITC and flow cytometric analysis. Conclusions Since hesperetin can induce apoptosis and reduce cell activity, it seems appropriate to be able to inhibit the growth of tumor cells.
E. Heidari Soureshjani, S. Vallian, S.f. Mirahmadi Babaheidari, F. Abasian,
Volume 14, Issue 2 (Summer 2017)
Abstract
Abstract
Background and Objectives
There is considerable phenotypic variation of Beta-thalassemia with common mutations whose understanding would facilitate the prevention of the specific syndromes. The aim of this study was to determine the common Beta-thalassemia mutations in Isfahan and Chaharmahal Bakhtiari province and thier relationship with blood parameters.
Materials and Methods
In this descriptive study, 10 ml venous blood samples were taken from 321 Beta thalassemia carriers having referred to the private Isfahan Center of Medical Genetics in 2016. For the confirmation of the diagnosis of Beta thalassemia in these patients, the indices of MCV and MCH with Mindary device and HbA1, HbA2, Hbf and RBC with the electrophoresis method were measured. c2, t-test and SPSS 22 were used in analyzing data.
Results
Mutation Fr36/37 (-T) in the population studied in Isfahan and Chaharmahal Bakhtiari with the frequency rates of 34 (26.35%) and 22 (32.35%) showed the highest in the studied mutations. About 80% of cases were detectable mutations in the beta globin gene in the people with HbA2 > 3.5 and in 100% of cases with MCH < 27 and MCV < 80.
Conclusions
Beta-thalassemia mutations among Chaharmahal Bakhtiari and Isfahan populations show diversity and wide distribution. Average mutations studied based on blood indices showed a wide variety. The results showed a positive correlation between beta-thalassemia mutations and red blood cells indices which can be effective in fast and efficient screening of these common mutations.
Dr. P. Fallah, M. Molaie, Dr. A. Ehsan Heidari, Dr. M. Soleimani, M. Jahedi Zargar, Dr. Ehsan Arefian, T. Madani, Dr. M. Asadi, Dr. B. Poopak,
Volume 15, Issue 1 (Spring 2018)
Abstract
Abstract
Background and Objectives
Acute lymphoblastic leukemia (ALL) is the most common malignancy in childhood. The prevalence of ALL is 1 to 4/7 per 100,000 population every year world-wide. Long non-coding RNAs (LncRNAs) are a group of non-coding RNAs involved in many biological processes, such as epigenetic changes, regulation of mRNA transcription and translation. Identification of their function can be helpful to understand the pathophysiology and treatment of ALL. One of the important LncRNAs in hematopoiesis regulation is HOTAIR (LncRNA HOX transcript antisense RNA). The purpose of this study was to determine the expression of HOTAIR LncRNA in newly diagnosed B-ALL patients.
Materials and Methods
In this cross-sectional descriptive study, 40 newly diagnosed B-ALL patients as well as 15 healthy individuals as control were given 1mL of bone marrow specimen with EDTA anticoagulant. RNA (patients and control) was extracted and Real-time quantitative PCR was used to examine the expression of LncRNA HOTAIR in ALL patients. The results was analyzed by REST 2009.
Results
The findings showed that expression of HOTAIR in patients with Acute Lymphoblastic Leukemia type B increased significantly as compared to healthy controls (p ˂ 0.05).
Conclusions
In conclusion, the increase expression of HOTAIR in acute lymphoblastic leukemia can be attributed to potential role of LncRNA HOTAIR in pathophysiology of the disease.
Farzaneh Taheri Ghahfarkhi, Dr. E. Tajbakhsh, E. Heidari Soureshjani,
Volume 15, Issue 4 (Winter 2018)
Abstract
Abstract
Background and Objectives
Viral hepatitis B and C are considered to be a medical problem for people with HIV infection. The importance of these infections is in the shared route of their transmission, and liver disease as a result of infection with HCV, HBV and HIV infection that can lead to death.
Materials and Methods
In this cross-sectional descriptive study, a total of 31 serum samples were prepared from patients referring to the Behavioral Diseases Counseling Center in Chaharmahal and Bakhtiari province who were diagnosed with HIV infection by Western blotting method; their consent was taken. The coinfection with HBV and HCV was investigated by PCR method. Data were analyzed by fisher exact test and prism7 software.
Results
The results of this study on serum samples of HIV-infected people showed the prevalence of HCV/HBV coinfection in HIV-positive individuals to be %3.22, the prevalence of HBV infection in HIV-positive individuals %4.45, and the prevalence of HCV infection in HIV infected ones %22.5.
Conclusions
According to the common routes of transmission of HCV, HBV, and HIV viruses, it is essential that all people who are HIV-positive or HIV-infected be screened for hepatitis B and C viruses by highly sensitive diagnostic methods.
Dr. R. Eslami, D. Heidary, A. Mehdipour, S. Heidari,
Volume 18, Issue 2 (Summer 2021)
Abstract
Abstract
Background and Objectives
The aim of this meta-analytical review was to compare the effect of different exercise training on plasma fibrinogen levels as well as to evaluate the acute and chronic response of fibrinogen to exercise.
Materials and Methods
In order to study the effects of exercise training on fibrinogen, a search was conducted in the databases of PubMed, Science Direct, Scopus, and Google Scholar. The search used identified keywords and reviewed articles published between 2010 and 2020. After the initial screening, full-text review and critical evaluation of the studies, the articles that met the inclusion criteria were analyzed. Finally, 22 studies including 33 trials with meta-analysis inclusion criteria were analyzed.
Results
The results of meta-analysis show that the acute response of fibrinogen to exercises is incremental, but the increase is not significant. On the other hand, the chronic response of fibrinogen to exercises is significantly reductive, which is related to aerobic exercise and high-intensity interval training and the response to anaerobic exercise is negligible.
Conclusions
In general, it can be said that exercise can be effective in modifying vascular fibrinogen levels. In various studies, in the acute response to exercise, there have been no change or increase in plasma fibrinogen levels but in the chronic response to exercise, a decrease in plasma fibrinogen levels has been reported. However, there is still a need for high quality studies with a sufficient sample size in this field.
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