Abstract

 Background and Objectives

 Clopidogrel is one of the most commonly prescribed drugs to prevent ischemic events following coronary syndromes or stent placement. The objective of this study was to evaluate the prevalence of the cytochrome P450 (CYP450) 2C19 enzyme (CYP2C19) genotypes in the Iranian population.

 Materials and Methods

 CYP2C19 (*1/*2/*3) variants were analyzed using Polymerase Chain Reaction-Restriction Length Polymorphism (PCR–RFLP) assays in a representative sample of 154 Iranian patients with ischemic heart disease in Peyvand Laboratory. 

 Results

 In this study, out of 154 Iranian patients the frequency rates of CYP2C19 *2 were 36 (23.4%) in heterozygous and 6 (3.9%) in homozygous forms 112 (72.7%) patients had the normal genotype.

 Conclusions

 FDA recommendations are more useful to be practiced in our country than other countries. Physicians should identify patients and advise them to consider other antiplatelet medications or alternative dosing strategies in poor metabolizers.

  Abstract

 Background and Objectives

 Micro RNAs are a class of small non-coding RNAs which have been recently shown to play a crucial role in major cellular processes such as development and differentiation through post-transcriptional regulation. The correlation of microRNA expression levels with the pathogenesis of some hematologic malignancies including acute myeloid leukemia is already known. The aim of this study was to investigate the expression of mir-150 and mir-155 in chronic myelogenous leukemia patients. miRNA expression level changes can be used for diagnosis, treatment and estimation of Minimal Residual Disease (MRD).

 Materials and Methods

 In this descriptive study, the expression of miR-150 and miR-155 in 25 newly diagnosed CML patients in chronic phase and 25 healthy subjects as the control all in Payvand Medical Laboratory were examined by Stem-loop RT-PCR and Real Time PCR techniques. For data analysis, Pearson correlation analysis was used.

 Results

 miR-150 and miR-155 were down-regulated in CML patients rather than in healthy subjects (0.512 and 0.2552, respectively). There was no correlation between the laboratory findings and the miR expression level. But miR-155 was inversely associated with age (p ≤ 0.01). 

 Conclusions

 In conclusion, the decreasing expression of miR-150 and miR-155 in chronic myelogenous leukemia is indicative of their induced expression in suppressing proliferation of cells in this disease. Other studies are also required to elucidate the exact role of microRNA by identifying their target genes.