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Showing 4 results for Dadashi

M. Dadashi, D. Bashash,
Volume 14, Issue 2 (Summer 2017)
Abstract

Abstract

Background and Objectives

PI3K/Akt pathway plays a key role in cell growth and proliferation. Constitutive activation of this pathway is detectable in 50-70% of patients with acute promyelocytic leukemia (APL). Previous studies showed that PI3K activity in APL cells is mainly due to overexpression of p110δ  isoform. In an effort to investigate the effect of PI3K inhibition in acute promyelocytic leukemia, APL-derived NB4 cells were subjected to different concentrations of Idelalisib, as a potent p110δ-specific inhibitor.

Materials and Methods

In this experimental study, we examined the effect of Idelalisib on the viability and metabolic activity of NB4 cells. Moreover, flowcytometery and quantitative RQ-PCR were applied to investigate both induction of apoptosis and transcriptional activity of apoptosis-related genes, respectively.

Results

The results revealed that Idelalisib not only decreased cell viability and metabolic activity in a dose- and time-dependent manner, but also induced cell death through the apoptotic pathway in NB4 cells. Our data also delineated that Idelalisib-induced apoptosis was mediated through induction of Bax and PUMA coupled with decreased expression of Mcl-1.

Conclusions

Based on P110δ activity in APL patients and the potent efficacy of Idelalisib in NB4 cells, it is tempting to suggest this inhibitor, as either single agent or in combination with common medications, for the treatment of patients with APL.


Dr. V. Mosmer, Dr. A.n. Moghaddam Dizaj Herik , B. Shokuei, S. Dadashi, Dr. M. Asghari Jafarabadi, Dr. Sh. Ardabilizadeh, Dr. A. Hossein Pour, Dr. P. Lotfinejad,
Volume 17, Issue 3 (Autumn 2020)
Abstract

Abstract
Background and Objectives
To encourage women to embark on blood donation, the opinions and factors that impede blood donation should be identified. The purpose of this study was to investigate factors affecting participation of women in blood donation.
 
Materials and Methods
In this cross-sectional study, the questionnaires were completed by 250 female staff of Tabriz University of Medical Sciences. Data were analyzed using Pearson correlation, one-way ANOVA and regression analysis.
 
Results
93.6% of people claimed blood donation is a humanitarian act, and 81.2% claimed "anemia" is barrier of blood donation. Regression analysis showed that there was a negative and significant relationship between knowledge and motivating factors that have impact on blood donation (p= 0.003), but barriers score did not show a significant correlation with women participation (p= 0.515). Based on the results of independent T-test, the relationship of marital status was not significant as compared with the knowledge  score (p = 0.834),  with  the  barriers score (p= 0.434), and with that of motivating factors (p = 0.615). Education level has not significantly correlated with the knowledge score (p = 0.510) but there was a significant negative correlation with barriers variable in blood donation (p = 0.17).
 
Conclusions 
According to the results, most of the participants in this study considered fear of anemia and fear of test results as barrier of blood donation. Therefore, establishing basic education and training programs to eliminate the wrong beliefs about blood donation would help encourage women as healthy sources of blood donors.
 
 

M. Rahimi Mofrad, Dr. F. Yari, Dr. M. Nikougoftar Zarif, M. Dadashi, Dr. A. Aghaie,
Volume 18, Issue 4 (Winter 2021)
Abstract

Abstract
Background and Objectives
Proliferation and differentiation of mesenchymal stem cells (MSCs) in culture media requires the presence of nutrient supplements such as bovine fetal serum (FBS), which increases the risk of prion/xenotic infections. Platelet lysates as a rich source of growth factors and cytokines can replace FBS in cell culture medium. This study aimed to compare the effect of platelet lysates derived from cord blood and peripheral blood on the proliferation and differentiation of MSCs.
 
Materials and Methods
In this experimental study, human MSCs were isolated from placental tissue and identified by flow-cytometry. 3 Three platelet bags were collected and platelet lysates were prepared by repeated freezing/thawing method. MSCs were cultured in different media and evaluated for their differentiation capacity, cultured in osteogenic and adipogenic differentiation media with Alizarin Red and Oil Red-O staining. TGF-β levels were assessed by ELISA. Statistical analysis was performed using SPSS22 software and One-Way ANOVA statistical test.
 
Results
The proliferation rate of MSCs in media containing Cord/Peripheral Blood Lysate (CBL or PBL) was not significantly different from that of media containing 5% FBS. Also, the rate of proliferation in the medium containing CBL plus cord blood serum was not significantly different from the medium containing 10% FBS. Also, the ability of MSCs’ differentiation into osteocytes and adipocytes in media containing CBL or PBL was well preserved.
 
Conclusions 
Platelet lysates can be a suitable alternative to animal serum in cell culture medium. In addition to the growth and proliferation of MSCs, these factors maintain the ability to differentiate into osteocytes and adipocytes in environments containing these factors.
 

Z. Tasbiti, Dr. M.s. Dadashi, Dr. A. Chegini, Dr. M. Zadsar,
Volume 20, Issue 4 (Winter 2023)
Abstract

Abstract
Background and Objectives
β-thalassemia patients need regular blood. Regular blood transfusion has side effects. The most common of which is FNHTR. One of the causes of FNHTR is the antibody production against HLA-II antigens. HLA polymorphism vary in different communities and may affect the susceptibility or resistance to FNHTR. In this study, the relationship between HLA-DR polymorphisms and development of FNHTR in multi-transfused thalassemia patients in Tehran province was investigated.

Materials and Methods
In this cross-sectional study, 88 ß-thalassemia major and intermediate patients with average age 38.63 ± 11.28 with 35 being male (39.8%) and 53 female (60.2%) were enrolled and for better results, 70 thalassemia patients with FNHTR were compared with 18 thalassemia patients without FNHTR. HLA-DR genotype was investigated by PCR-SSP method. Data were analyzed by chi-squared and pearson test.

Results
The results of PCR-SSP showed that HLA-DRB1*11 allele had the highest prevalence (26.7%) in the total study population. No statistically significant correlation between HLA-DR alleles and development of FNHTR was observed. Although HLA-DRB1*10 as a possible genetic marker may play a role in protecting against FNHTR (p = 0.049).

Conclusions 
In this study, no significant association between HLA-DR allele frequency and FNHTR was found. Although alleles HLA-DRB1*03, HLA-DRB1*10, HLA-DRB1*13 and HLA-DRB1*16 have higher prevalence in the no FNHTR group, but only in the case of HLA-DRB1*10 this relationship was significant and was considered as possible genetic markers against development of FNHTR. Further studies investigating HLA-II allelic levels in larger populations may provide more deep data on this association.
 

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