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Showing 2 results for Arefian
P. Fallah, Dr. H. Timori Naghadeh, Dr. M. Soleimani, Dr. N. Amirizadeh, Dr. B. Poopak, Dr. Gh. Toogeh, Dr. E. Arefian, Sh. Bolouri, T. Golkar, Z. Pirmohammad Jamaat,
Volume 12, Issue 3 (Autumn 2015)
Abstract
Abstract Background and Objectives Micro RNAs are a class of small non-coding RNAs which have been recently shown to play a crucial role in major cellular processes such as development and differentiation through post-transcriptional regulation. The correlation of microRNA expression levels with the pathogenesis of some hematologic malignancies including acute myeloid leukemia is already known. The aim of this study was to investigate the expression of mir-150 and mir-155 in chronic myelogenous leukemia patients. miRNA expression level changes can be used for diagnosis, treatment and estimation of Minimal Residual Disease (MRD). Materials and Methods In this descriptive study, the expression of miR-150 and miR-155 in 25 newly diagnosed CML patients in chronic phase and 25 healthy subjects as the control all in Payvand Medical Laboratory were examined by Stem-loop RT-PCR and Real Time PCR techniques. For data analysis, Pearson correlation analysis was used. Results miR-150 and miR-155 were down-regulated in CML patients rather than in healthy subjects (0.512 and 0.2552, respectively). There was no correlation between the laboratory findings and the miR expression level. But miR-155 was inversely associated with age (p ≤ 0.01). Conclusions In conclusion, the decreasing expression of miR-150 and miR-155 in chronic myelogenous leukemia is indicative of their induced expression in suppressing proliferation of cells in this disease. Other studies are also required to elucidate the exact role of microRNA by identifying their target genes.
Dr. P. Fallah, M. Molaie, Dr. A. Ehsan Heidari, Dr. M. Soleimani, M. Jahedi Zargar, Dr. Ehsan Arefian, T. Madani, Dr. M. Asadi, Dr. B. Poopak,
Volume 15, Issue 1 (Spring 2018)
Abstract
Abstract
Background and Objectives
Acute lymphoblastic leukemia (ALL) is the most common malignancy in childhood. The prevalence of ALL is 1 to 4/7 per 100,000 population every year world-wide. Long non-coding RNAs (LncRNAs) are a group of non-coding RNAs involved in many biological processes, such as epigenetic changes, regulation of mRNA transcription and translation. Identification of their function can be helpful to understand the pathophysiology and treatment of ALL. One of the important LncRNAs in hematopoiesis regulation is HOTAIR (LncRNA HOX transcript antisense RNA). The purpose of this study was to determine the expression of HOTAIR LncRNA in newly diagnosed B-ALL patients.
Materials and Methods
In this cross-sectional descriptive study, 40 newly diagnosed B-ALL patients as well as 15 healthy individuals as control were given 1mL of bone marrow specimen with EDTA anticoagulant. RNA (patients and control) was extracted and Real-time quantitative PCR was used to examine the expression of LncRNA HOTAIR in ALL patients. The results was analyzed by REST 2009.
Results
The findings showed that expression of HOTAIR in patients with Acute Lymphoblastic Leukemia type B increased significantly as compared to healthy controls (p ˂ 0.05).
Conclusions
In conclusion, the increase expression of HOTAIR in acute lymphoblastic leukemia can be attributed to potential role of LncRNA HOTAIR in pathophysiology of the disease.
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