Scientific Journal of

1. Sies H. What is oxidative stress?  Oxidative stress and vascular disease. Volume 224 of the series Developments in Cardiovascular Medicine. Philadelphia: Springer; 2000. p. 1-8.
2. Sies H. Oxidative stress: from basic research to clinical application. Am J Med 1991; 91(3C): 31S-38S.
3. de Groot H. Reactive oxygen species in tissue injury. Hepatogastroenterology 1994; 41(4): 328-32.
4. Scandalios JG. Oxidative stress: molecular perception and transduction of signals triggering antioxidant gene defenses. Braz J Med Biol Res 2005; 38(7): 995-1014.
5. Sailaja Y, Baskar R, Saralakumari D. The antioxidant status during maturation of reticulocytes to erythrocytes in type 2 diabetics. Free Radic Biol Med 2003; 35(2): 133-9.
6. Ogunro P, Ogungbamigbe T, Muhibi M. The influence

1. Zelko IN, Mariani TJ, Folz RJ. Superoxide dismutase multigene family: a comparisonof the CuZn-SOD (SOD1), Mn-SOD (SOD2), and EC-SOD (SOD3) gene structures, evolution, and expression. Free Radic Biol Med 2002; 33(3): 337-49.
2. Zoroglu SS, Armutcu F, Ozen S, Gurel A, Sivasli E, Yetkin O, et al. Increased oxidative stress and altered activities of erythrocyte free radical scavenging enzymes in autism. Eur Arch Psychiatry Clin Neurosci 2004; 254(3): 143-7.
3. Levander OA. A global view of human selenium nutrition. Annu Rev Nutr 1987; 7(1): 227-50.
4. Williams K, Frayne J, Hall L. Expression of extracellular glutathione peroxidase type 5 (GPX5) in the rat male reproductive tract. Mol Hum Reprod 1998; 4(9): 841-8.
5. Roxborough HE, Mercer C, McMaster D, Maxwell AP, Young IS. Plasma glutathione peroxidase activity is reduced in haemodialysis patients. Nephron 1999; 81(3): 278-83.
6. Slabber P. Perioperative Transfusion Triggers; 2013. Available from: http://anaesthetics.ukzn.ac.za/Libraries/Documents2011/P_Slabber_Perioperative_20RBC_transfusion_practice_1.sflb.ashx.
7. Bode AP. Platelet activation may explain the storage lesion in platelet concentrates. Blood Cells 1989; 16(1): 109-25; discussion 125-6.
8. Alamdari DH, Ghayour-Mobarhan M, Tavallaie S, Parizadeh MR, Moohebati M, Ghafoori F, et al. Prooxidant–antioxidant balance as a new risk factor in patients with angiographically defined coronary artery disease. Clin Biochem 2008; 41(6): 375-80.
9. Jóźwik M, Jóźwik M, Jóźwik M, Szczypka M, Gajewska J, Laskowska-Klita T. Antioxidant defence of red blood cells and plasma in stored human blood. Clin Chim Acta 1997; 267(2): 129-42.
10. Kriebardis AG, Antonelou MH, Stamoulis KE, Economou-Petersen E, Margaritis LH, Papassideri IS. Membrane protein carbonylation in non-leukodepleted CPDA-preserved red blood cells. Blood Cells Mol Dis 2006; 36(2): 279-82.
11. Shazia Q, Mohammad Z, Rahman T, Shekhar HU. Correlation of oxidative stress with serum trace element levels and antioxidant enzyme status in Beta thalassemia major patients: a review of the literature. Anemia 2012; 2012: 270923.
12. Rinalducci S, Ferru E, Blasi B, Turrini F, Zolla L. Oxidative stress and caspase-mediated fragmentation of cytoplasmic domain of erythrocyte band 3 during blood storage. Blood Transfus 2012; 10 Suppl 2: s55-62.
13. Chaudhary R, Likidlilid A, Peerapatdit T, Tresukosol D, Srisuma S, Ratanamaneechat S, et al. Apolipoprotein E gene polymorphism: effects on plasma lipids and risk of type 2 diabetes and coronary artery disease. Cardiovasc Diabetol 2012; 11: 36.
14. Ghayour-Mobarhan M, Alamdari D, Moohebati M, Sahebkar A, Nematy M, Safarian M, et al. Determination of pro-oxidant–antioxidant balance after acute coronary syndrome using a rapid assay: a pilot study. Angiology 2009; 60(6): 657-62.
15. Parizadeh SM, Azarpazhooh MR, Moohebati M, Nematy M, Ghayour-Mobarhan M, Tavallaie S, et al. Simvastatin therapy reduces prooxidant-antioxidant balance: results of a placebo-controlled cross-over trial. Lipids 2011; 46(4): 333-40.
16. Korgun DK, Bilmen S, Yesilkaya A. Alterations in the erythrocyte antioxidant system of blood stored in blood bags. Res Commun Mol Pathol Pharmacol 2000; 109(5-6): 357-63.
17. Aslan R, Sekeroğlu M, Tarakçioğlu M, Köylü H. Investigation of malondialdehyde formation and antioxidant enzyme activity in stored blood. Haematologia (Budap) 1996; 28(4): 233-7.
18. Bartosz G, Tannert C, Fried R, Leyko W. Superoxide dismutase activity decreases during erythrocyte aging. Experientia 1978; 34(11): 1464.
19. Lippa S, Forni F, Candido A, Aureli V, Mango G. Oxidative stress of red blood cells stored for transfusion use. Folia Haematol Int Mag Klin Morphol Blutforsch 1989; 117(1): 105-10.
20. Marjani A, Mansoorian A, Joshaghani H, Heydari K, Sarikhani A. The Alterations of Plasma Iipid Peroxidation and erythrocyte Superoxide Dismutase and Glutathione Peroxidase Enzyme Activities During Storage of Blood. Medical Laboratory Journal 2007; 1(1): 5-11. [Article in Farsi]
21. Gultekin F, Akdogan M, Altuntas I, Delibas N, Kaptanagasi M. Changes in erythrocyte lipid peroxidation and antioxidant potential during storage of blood and protective effect of melatonin. J Biochem. 2000; 25: 83-91.

 

 

Oxidative stress changes in blood bags in consecutive
weeks after donation
 
Barzegar S.¹, Nadali F.¹, Pourfathollah A.A.^2,3, Abbaspour A.R.⁴, Farokhinia S.⁵, Shiravand Y.^1
 
 
1School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran
²Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
³Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
⁴School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
⁵Emam Ali Hospital, North Khorasan University of Medical Sciences, Bojnourd, Iran
 
 
Abstract
Background and Objectives
The oxidative stress destroys the red blood cells during preservation. The awareness about the stress oxidative process is important for the safety of blood bags and patients. The aim of the study was to evaluate the pro-oxidant-antioxidant balance (PAB) and superoxide dismutase (SOD) and glutathione peroxidase (GPX) enzymes in blood bags in the weeks after donation.
 
Materials and Methods
In a descriptive study, a number of 100 blood bags were used for the blood donors between 18 to 57 years of age (the mean age of 35.5) in Bojnourd Blood Center. The blood bags were kept in standard conditions for 6 weeks and the adequate samples were taken for test. The test included PAB, SOD, and GPX and the repeated measures analysis was performed.
 
Results
The pro-oxidant-antioxidant balance had a significant increase (p < 0.000). Enzymes like SOD and GPX had a significant decrease (p < 0.000).
 
Conclusions
Results showed that pro-oxidant-antioxidant balance and antioxidant enzyme activities changed particularly in the first two weeks. Therefore, it is suggested that for the diseases related with oxidative stress such as cardiovascular diseases, diabetes, and cancers, patients receive blood stored less than two weeks.
 
Key words: Superoxide Dismutase, Glutathione Peroxidase, Blood Donation

 
 
 
 
 
 
Received:   3  Mar 2015
Accepted: 28 Oct  2015
 
 

 

Correspondence: Nadali F., PhD in Hematology & Blood Banking. Associate Professor of School of Allied Medical Sciences, Tehran University of Medical Sciences.
P.O.Box: 14177-44361, Tehran, Iran. Tel: (+9821) 88982815; Fax: (+9821) 88983037
E-mail: Nadalifa@yahoo.com