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:: Volume 12, Issue 1 (Spring 2015) ::
Sci J Iran Blood Transfus Organ 2015, 12(1): 13-22 Back to browse issues page
Expansion of human cord blood hematopoietic stem/progenitor cells in three-dimensional nano fibrous collagen coated scaffold
S.H. Mousavi , S. Abroun , M. Soleimani , S.J. Mowla , Gh. Tamadon
Keywords: Key words : Cord Blood Stem Cell Transplantation, Hematopoietic Stem Cells, Tissue Engineering
Full-Text [PDF 375 kb]   (1779 Downloads)     |   Abstract (HTML)  (7265 Views)
Type of Study: Research | Subject: Stem cells
Published: 2015/04/7
Full-Text:   (1787 Views)
References :  
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Original  Article
 
 
 
 
Sci J Iran Blood Transfus Organ 2015; 12(1): 13-22
 
 

 

Expansion of human cord blood hematopoietic
stem/progenitor cells in three-dimensional nano fibrous
 collagen coated scaffold
 
Mousavi S.H.1, Abroun S.1, Soleimani M.1, Mowla S.J.2, Tamadon Gh.1,3
 
 
1Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2Department of Genetic, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
3School of Allied Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
 
 
Abstract
Background and Objectives
The allogeneic hematopoietic stem cell transplantation is used for the treatment of some hematological and non-hematological disorders, but the lack of suitable donors for many patients limits the use of this source therapy. Umbilical cord blood (UCB) is an alternative source of hematopoietic stem cell (HSC) transplantation. Despite all advantage lower cell doses is major obstacle in cord blood transplantation. Exvivo expansion of HSC is an alternative way to overcome this problem.
 
Materials and Methods
In this experimental study, Polycaprolactone (PCL) scaffold coated with nano fibrous collagen compared to routine cell culture system is used for cell culture. Total cells, CD34 cell, CFC assay, and CXCR-4 expression was performed.
 
Results
Our findings demonstrated that the total cell expansion and CD34+ cells in 3 dimensional (3D) scaffold compared to routine culture system (2D)  was higher (p< 0.05).  Also total colony cells in 3D scaffold was higher than 2D cell culture system and statically significant (p< 0.05). Higher CXCR-4 expression in 3D compared to 2D showed better homing of cells cultured in 3D scaffold (p< 0.05).
 
Conclusions
PCL scaffold coated with nano fibrous collagen was a proper cell culture system compared to 2D for cell expansion.
 
Key words: Cord Blood Stem Cell Transplantation, Hematopoietic Stem Cells, Tissue Engineering
 
 
 
 
 
 
Received:  30 Aug 2014  
Accepted: 11  Nov 2014
 
 
 

Correspondence: Abroun S., PhD of Hematology. Associate Professor of Faculty of Medical Sciences, Tarbiat Modares University.  
P.O.Box: 14155-331, Tehran, Iran. Tel: (+9821) 82883860; Fax : (+9821) 82884507
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Mousavi S, Abroun S, Soleimani M, Mowla S, Tamadon G. Expansion of human cord blood hematopoietic stem/progenitor cells in three-dimensional nano fibrous collagen coated scaffold . Sci J Iran Blood Transfus Organ 2015; 12 (1) :13-22
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Volume 12, Issue 1 (Spring 2015) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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