Volume 11, Issue 1 (Spring 2014 2015)                   Sci J Iran Blood Transfus Organ 2015, 11(1): 32-40 | Back to browse issues page

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Sakhteh M, Poopak B, Amirizadeh N, Shamshiri A, Bagheri A, Faranoush M. Association of 4G/5G polymorphism in Plasminogen activator inhibitor-inhibitor (PAI-1) gene with thrombosis in coronary arteries. Sci J Iran Blood Transfus Organ 2015; 11 (1) :32-40
URL: http://bloodjournal.ir/article-1-853-en.html
Association of 4G/5G polymorphism in Plasminogen activator inhibitor-inhibitor (PAI-1) gene with thrombosis in coronary arteries
M. Sakhteh , B. Poopak , N. Amirizadeh , A.R. Shamshiri , A.H. Bagheri , M. Faranoush *
Keywords: Key words: Coronary Thrombosis, Myocardial Infarction, Coronary Artery Disease, PAI-1
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Sci J Iran Blood Transfus Organ 2014; 11(1): 32-40
 
Original Article
 
 

Association of 4G/5G polymorphism in Plasminogen
 activator inhibitor-inhibitor (PAI-1) gene with
thrombosis in coronary arteries
 
Sakhteh M.1, Poopak B.2, Amirizadeh N.1, Shamshiri A.R.3, Bagheri A.H.4, Faranoush M.5
 
 
1Blood Transfusion Research Center, High Institute for Education and Research in Transfusion Medicine, Tehran, Iran
2School of Paramedicine, Tehran Medical Branch of Islamic Azad University, Tehran, Iran
3Faculty of Dendistry, Tehran University of Medical Sciences, Tehran, Iran
4Modares Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran
 
 
 
Abstract
Background and Objectives
Several hereditary and acquired risk factors for thrombosis are known. Among the genetic factors, PAI-1 4G/5G polymorphism can be noted. This study was done to investigate the association of 4G/5G polymorphism in PAI-1 gene and thrombosis in coronary arteries.
 
Materials and Methods
Sixty one patients with the history of thrombosis in coronary arteries and 92 healthy blood donors participated as the control in our study. After DNA extraction from leucocytes based on the selective detergent-mediated DNA precipitation from crude lysate, PCR was performed using ARMS technique. Single and multivariate analyses were applied to adjust for potentially confounding factors using SPSS 19 software. The data were also compared with those of the other similar studies.
 
Results
The results showed 61 patients with history of coronary artery thrombosis for PAI-1 with the values of 24.6%, 45.9%, and 29.5% for 4G/4G, 4G/5G, and 5G/5G, respectively; the values for 92 healthy blood donors were evaluated to be 20.7%, 42.2%, and 37% in order. The polymorphism studied was not significantly different between cases and controls. Single and multivariate analyses show a significant difference for the conventional risk factors for coronary artery disease between patients and healthy controls (P value: 0.001).
 
Conclusions 
We found no association between arterial thrombosis and the 4G/4G genotype for PAI-1 gene in Iranian population of the current study.
 
Key words: Coronary Thrombosis, Myocardial Infarction, Coronary Artery Disease, PAI-1
 
 
 
Received: 13 May 2013
Accepted: 11 Aug 2013
 
 

Correspondence: Faranoush M., MD. Pediatrics Hematologist-Oncologist. Associate Professor of Rasoul Akram Hospital, Iran University of Medical Sciences.
P.O.Box: 14166-1157, Tehran, Iran. Tel: (+9821) 88605151; Fax: (+9821) 88601580
E-mail:
faranoush47@yahoo.com
Type of Study: Research | Subject: Hematology
Published: 2014/03/16
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