Volume 10, Issue 3 (Autumn 2013)
Sci J Iran Blood Transfus Organ 2013, 10(3): 289-296 |
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Hashemi Tayer A, Amirizadeh N, Almasi Hashiani A, Kamravan M, Mohammadi M. A new fibrin sealant: hemostatics evaluation in the animal model. Sci J Iran Blood Transfus Organ 2013; 10 (3) :289-296
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URL: http://bloodjournal.ir/article-1-798-en.html
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A new fibrin sealant: hemostatics evaluation
in the animal model
Hashemi Tayer A.1, Amirizadeh N.2, Almasi-Hashiani A.3, Kamravan M.4, Mohammadi M.H.2
1School of Paramedicine, Arak University of Medical Sciences, Arak, Iran
2Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
3School of Health, Arak University of Medical Sciences, Arak, Iran
4Arak University of Medical Sciences, Arak, Iran
Abstract
Background and Objectives
Fibrin based sealants are frequently used to arrest blood loss in surgery. The aim of this study was to prepare fibrin-based sealants from thrombin and fibrinogen and evaluate the hemostatic efficacy of the fibrin sealant in a rabbit animal model.
Materials and Methods
In this experimental study, plasma was prepared with the apheresis method. Fibrinogen was prepared by mixing the cryoprecipitate with protamine sulfate and its concentration was assayed with an ELISA method. The fibrin sealant was applied to the controlled animal liver incisions; then, the time of bleeding and the volume of blood loss were measured. Blood loss and time of bleeding were also evaluated in heparinized rabbits.
Results
The fibrinogen concentration precipitated with protamine sulfate was measured as being 71 ± 6 mg/ml. The thrombin mixed with fibrinogen had clot time of 5 ± 0.6 seconds. The application of the fibrin sealant significantly reduced the time of bleeding (8.8 ± 2 sec) and blood loss (1.05 ± 0.4 gr) of the rabbit liver incisions compared with the control (643 ± 96 sec, 6.9 ± 1.2 gr)(p<0.001). Despite the use of the fibrin sealant, the blood loss and the time of bleeding were greater, though not statistically significant, in the heparinized rabbits than in the nonheparinized rabbits (p= 0.2).
Conclusions
The fibrin sealant prepared by the precipitation of fibrinogen and thrombin offers a novel therapeutic approach to obtain autologus sealants for clinical use.
Key words: Animal model, Fibrin sealant, Hemostatics
Received: 31 Jul 2012
Accepted: 22 Oct 2012
Correspondence: Hashemi Tayer A., MSc of Hematology. Instructor of School of Paramedicine, Arak University of Medical Sciences.
P.O.Box: 12456-72334, Arak, Iran. Tel: (+98861) 4173526; Fax: (+98861) 4173526
E-mail: hashemiakbar@arakmu.ac.ir
Full-Text: (3709 Views)
References :
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- Traver MA, Assimos DG. New generation tissue sealants and hemostatic agents: innovative urologic applications. Rev Urol 2006; 8(3): 104-11.
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A new fibrin sealant: hemostatics evaluation
in the animal model
Hashemi Tayer A.1, Amirizadeh N.2, Almasi-Hashiani A.3, Kamravan M.4, Mohammadi M.H.2
1School of Paramedicine, Arak University of Medical Sciences, Arak, Iran
2Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
3School of Health, Arak University of Medical Sciences, Arak, Iran
4Arak University of Medical Sciences, Arak, Iran
Abstract
Background and Objectives
Fibrin based sealants are frequently used to arrest blood loss in surgery. The aim of this study was to prepare fibrin-based sealants from thrombin and fibrinogen and evaluate the hemostatic efficacy of the fibrin sealant in a rabbit animal model.
Materials and Methods
In this experimental study, plasma was prepared with the apheresis method. Fibrinogen was prepared by mixing the cryoprecipitate with protamine sulfate and its concentration was assayed with an ELISA method. The fibrin sealant was applied to the controlled animal liver incisions; then, the time of bleeding and the volume of blood loss were measured. Blood loss and time of bleeding were also evaluated in heparinized rabbits.
Results
The fibrinogen concentration precipitated with protamine sulfate was measured as being 71 ± 6 mg/ml. The thrombin mixed with fibrinogen had clot time of 5 ± 0.6 seconds. The application of the fibrin sealant significantly reduced the time of bleeding (8.8 ± 2 sec) and blood loss (1.05 ± 0.4 gr) of the rabbit liver incisions compared with the control (643 ± 96 sec, 6.9 ± 1.2 gr)(p<0.001). Despite the use of the fibrin sealant, the blood loss and the time of bleeding were greater, though not statistically significant, in the heparinized rabbits than in the nonheparinized rabbits (p= 0.2).
Conclusions
The fibrin sealant prepared by the precipitation of fibrinogen and thrombin offers a novel therapeutic approach to obtain autologus sealants for clinical use.
Key words: Animal model, Fibrin sealant, Hemostatics
Received: 31 Jul 2012
Accepted: 22 Oct 2012
Correspondence: Hashemi Tayer A., MSc of Hematology. Instructor of School of Paramedicine, Arak University of Medical Sciences.
P.O.Box: 12456-72334, Arak, Iran. Tel: (+98861) 4173526; Fax: (+98861) 4173526
E-mail: hashemiakbar@arakmu.ac.ir
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