Volume 10, Issue 2 (Summer 2013)
Sci J Iran Blood Transfus Organ 2013, 10(2): 103-111 |
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Sabaghi F, Shams Asanjan K, Movasaghpour A, Amirizadeh N, Nikougoftar M, Bagheri N et al . Proliferation promoting and apoptosis inhibiting effect of aminated PS nanofibers on cord blood hematopoietic stem cells. Sci J Iran Blood Transfus Organ 2013; 10 (2) :103-111
URL: http://bloodjournal.ir/article-1-783-en.html
URL: http://bloodjournal.ir/article-1-783-en.html
F. Sabaghi 
, K. Shams Asanjan * , A.A. Movasaghpour , N. Amirizadeh , M. Nikougoftar , N. Bagheri , P. Abbasi
, K. Shams Asanjan * , A.A. Movasaghpour , N. Amirizadeh , M. Nikougoftar , N. Bagheri , P. Abbasi
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| Abstract (HTML) (11889 Views)
Proliferation promoting and apoptosis inhibiting effect of aminated PS nanofibers on cord blood hematopoietic
stem cells
Sabaghi F.1, Shams Asanjan K.2,3, Movasaghpour Akbari A.A. 1, Amirizadeh N. 2, Nikougoftar M. 2,
Bagheri N. 2, Abbasi P. 1
1Hematology Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
3Tabriz Educational Regional Blood Transfusion Center, Tabriz, Iran
Abstract
Background and Objectives
Umbilical cord blood is a promising alternative source of hematopoietic stem/progenitor cells (HSPCs) for allogeneic hematopoietic stem cell transplantation. Although the use of UCB for transplantation has plentiful advantages in comparison with other cell sources, the low number of HSPCs led to cell delayed recovery. Therefore, ex vivo expansion of HSPC before transfusion could induce faster cell recovery.
Materials and Methods
CD34 positive cells were positively enriched using MidiMACS system and cultured on aminated PS nanofibers, aligned PCLs, and random PCLs. Cell expansion and quantification of cell apoptosis were carried out in expanded cell populations in different conditions by the flowcytometric method.
Results
This study indicated significantly increased expansion of CD34+ cells and declined apopotosis in PS-cultured cells compared to the same cells on 2-D control culture system (p< 0.05). There were no significantly differences in expansion of CD34+ cells on aligned and random PCL scaffolds than conventional system.
Conclusions
This study demonstrated that aminated PS nanofibers has positive effects on the expansion of CD34+ cells compared to 2D culture system by increasing cell expansion and cell apopotosis inhibition. However, aligned and random PCL nanofibers did not show supportive effects on cell proliferation.
Key words: Cord Blood, Hematopoietic Stem Cells, CD34 Antigens
Received: 13 Oct 2011
Accepted: 7 Oct 2012
Correspondence: Shams Asanjan K., PhD of Hematology and Blood Banking. Assistant Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 51335, Tabriz , Iran. Tel: (+98411) 2871515; Fax: (+98411) 2871515
E-mail: k.shams@ibto.ir
Full-Text: (1657 Views)
References:
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Biophys Acta 2002; 1592(3): 313-21. - Nikougoftar Zarif M, Soleimani M, Abolghasemi H, Amirizade N, Abroun S, Kaviani S. The High Yield Expansion and Megakaryocytic Differentiation of Human Umbilical Cord Blood CD133(+) Cells. Cell J 2011; 13(3): 173-8
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Original Article
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Sci J Iran Blood Transfus Organ 2013; 10(2): 103-111 |
Proliferation promoting and apoptosis inhibiting effect of aminated PS nanofibers on cord blood hematopoietic
stem cells
Sabaghi F.1, Shams Asanjan K.2,3, Movasaghpour Akbari A.A. 1, Amirizadeh N. 2, Nikougoftar M. 2,
Bagheri N. 2, Abbasi P. 1
1Hematology Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
3Tabriz Educational Regional Blood Transfusion Center, Tabriz, Iran
Abstract
Background and Objectives
Umbilical cord blood is a promising alternative source of hematopoietic stem/progenitor cells (HSPCs) for allogeneic hematopoietic stem cell transplantation. Although the use of UCB for transplantation has plentiful advantages in comparison with other cell sources, the low number of HSPCs led to cell delayed recovery. Therefore, ex vivo expansion of HSPC before transfusion could induce faster cell recovery.
Materials and Methods
CD34 positive cells were positively enriched using MidiMACS system and cultured on aminated PS nanofibers, aligned PCLs, and random PCLs. Cell expansion and quantification of cell apoptosis were carried out in expanded cell populations in different conditions by the flowcytometric method.
Results
This study indicated significantly increased expansion of CD34+ cells and declined apopotosis in PS-cultured cells compared to the same cells on 2-D control culture system (p< 0.05). There were no significantly differences in expansion of CD34+ cells on aligned and random PCL scaffolds than conventional system.
Conclusions
This study demonstrated that aminated PS nanofibers has positive effects on the expansion of CD34+ cells compared to 2D culture system by increasing cell expansion and cell apopotosis inhibition. However, aligned and random PCL nanofibers did not show supportive effects on cell proliferation.
Key words: Cord Blood, Hematopoietic Stem Cells, CD34 Antigens
Received: 13 Oct 2011
Accepted: 7 Oct 2012
Correspondence: Shams Asanjan K., PhD of Hematology and Blood Banking. Assistant Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 51335, Tabriz , Iran. Tel: (+98411) 2871515; Fax: (+98411) 2871515
E-mail: k.shams@ibto.ir
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