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:: Volume 10, Issue 2 (Summer 2013) ::
Sci J Iran Blood Transfus Organ 2013, 10(2): 112-121 Back to browse issues page
Evaluation of expansion and differentiation of cord blood hematopoietic cells expanded in suspension bioreactor with vertical agitation
N. Shayan Asl , M. Ebrahimi , B. Beiki , E. Janzamin , P. Bani Ardalan
Keywords: Key words: Cord Blood, Hematopoietic Stem Cells, Bioreactors
Full-Text [PDF 583 kb]   (2442 Downloads)     |   Abstract (HTML)  (13928 Views)
Type of Study: Research | Subject: Stem cells
Published: 2013/08/24
Full-Text:   (1653 Views)
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Original Article
 
 
 
 
Sci J Iran Blood Transfus Organ 2013; 10(2): 112-121
 
 

Evaluation of expansion and differentiation of
cord blood hematopoietic cells expanded in suspension
bioreactor with vertical agitation
 
 
Shayan Asl N.1, Ebrahimi M.1,2, Beiki B.2, Janzamin E.1, Bani Ardalan P.2
 
 
 
1Department of Regenerative Medicine at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
2Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
 
 
 
Abstract
Background and Objectives
Umblical cord blood (UCB) hematopoietic stem cells having high cell counts are suitable candidates for benign and malignant disease transplantation but often UCBs have low counts. In this study, ex vivo expansion of these cells was performed using suspension bioreactor with vertical agitation.
 
Materials and Methods
UCB Mononuclear cells were cultured with 1.07 × 106 cells/ml density in total 206 ml of culture medium in the suspension bioreactor with vertical agitator; and the static group with same density was cultured in T-flasks for 14 days. At the days 0, 3, 7 and 14 of culture, sampling was performed for further analysis.
 
Results
During the 3 days of culture, differentiated cells were lost and then the number of cells increased again from 0.42 × 106 cells/ml up to 1.2 × 106 cells/ml and from 0.6 × 106 up to 2.9 × 106 in bioreactor and static culture, respectively. The percentage of CD34+ cells and colony forming potential significantly increased in bioreactor.
 
Conclusions 
Hematopoietic stem cells in the bioreactor while retaining their self-potential are expanded. However, due to the increasing shear stress in the environment, the total number of cells did not increase. Therefore, further studies are required.
 
Key words: Cord Blood, Hematopoietic Stem Cells, Bioreactors
 
 
 
 
Received: 20 May 2012
Accepted:19 Sep  2012
 
 

Correspondence: Ebrahimi M., PhD of Immunology. Assistant Professor of Department of Regenerative Biomedicine at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR.
P.O.Box: 19395-4644, Tehran, Iran. Tel: (+9821) 23562000; Fax: (+9821) 22413790
E-mail:
mebrahimi@royaninstitute.org
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Shayan Asl N, Ebrahimi M, Beiki B, Janzamin E, Bani Ardalan P. Evaluation of expansion and differentiation of cord blood hematopoietic cells expanded in suspension bioreactor with vertical agitation. Sci J Iran Blood Transfus Organ 2013; 10 (2) :112-121
URL: http://bloodjournal.ir/article-1-765-en.html
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Volume 10, Issue 2 (Summer 2013) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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