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Effect of epinephrine on the CXCR4 expression of human cord blood derived CD34+ hematopoietic stem cells
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F. Saba   , E. Roshandel , M. Soleimani , A. Atashi , A. Gharehbaghian , S. Abroun , S. Kaviani |
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| Keywords: Key words : Hematopoietic Stem Cells, Epinephrine, Granulocyte Colony-Stimulating Factor |
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Type of Study: Research |
Subject:
Stem cells
Published: 2014/12/31
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Full-Text: (1644 Views) |
References :
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Sci J Iran Blood Transfus Organ 2015; 11(4): 271-280
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Effect of epinephrine on the CXCR4 expression of
human cord blood derived CD34+ hematopoietic
stem cells
Saba F.1, Roshandel E.2, Soleimani M.1, Atashi A.1, Gharehbaghian A.2,3,
Abroun S.1, Kaviani S.1
1Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2Faculty of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3Pediatric Congenital Hematologic Disorders Research Center of Shahid Beheshti University of Medical
Sciences, Tehran, Iran
Abstract
Background and Objectives
Recently, circulating hematopoietic stem cells (HSC) have been used for the treatment of most types of leukemia. However, the G-CSF mechanism has not been known yet but it is believed that G-CSF is mostly effective by its indirect functions. The suppression of the nervous system affects G-CSF induced mobilization of HSC. The main gene involved in mobilization is CXCR4 that ligand to SDF-1. So, this study investigates the effect of the main catecholamine of nervous system-Epinephrin- on CXCR4 expression.
Materials and Methods
In this experimental study, isolated cord blood CD34 + cell of 20 healthy newborn with MACs columns treated with 10 μM Epinephrine and 1 μM Proporanolol. Expression of CXCR4 has been investigated at 1, 3 and 5 hours with qRT-PCR. Receptors of beta adrenergic expression have been studied with RT-PCR.
Results
Beta adrenergic receptors are expressed on CD34 + cells. Epinephrine led to significantly increased CXCR4 in hours 1 (3.2 ± 0.5), 3 (2.4 ± 0.4) and 5 (2.2 ± 0.4 ). Cells treated with propranolol, return increased CXCR4 induced by epinephrine.
Conclusions
It can be concluded that G-CSF affects the expression of CXCR4 through increasing secretion of epinephrine in bone marrow with on beta adrenergic receptors. Cells with increased CXCR4 mobilized to release SDF-1 in peripheral blood.
Key words: Hematopoietic Stem Cells, Epinephrine, Granulocyte Colony-Stimulating Factor,
CXCR4 Receptor
Received: 21 May 2013
Accepted: 10 Jun 2014
Correspondence: Soleimani M., PhD of Hematology. Associate Professor of Faculty of Medical Sciences, Tarbiat Modares University.
P.O.Box: 14115-111, Tehran, Iran. Tel: (+9821) 88011001; Fax: (+9821) 88013030
E-mail: soleim_m@modares.ac.ir
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Saba F, Roshandel E, Soleimani M, Atashi A, Gharehbaghian A, Abroun S et al . Effect of epinephrine on the CXCR4 expression of human cord blood derived CD34+ hematopoietic stem cells . Sci J Iran Blood Transfus Organ 2015; 11 (4) :271-280 URL: http://bloodjournal.ir/article-1-753-en.html
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