[Home ] [Archive]   [ فارسی ]  
:: Main :: About us :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 8, Issue 2 (Summer 2011) ::
Sci J Iran Blood Transfus Organ 2011, 8(2): 104-114 Back to browse issues page
Quantification of fetomaternal hemorrhage: a compariative study of the HbF dual staining and RhD single staining by flow cytometry
F. Khoshnaghsh, M. Kheirandish , M.R. Deyhim
Abstract:   (18642 Views)

  Abstract

 Background and Objectives

 The quantification of fetal cells in the maternal circulation is an important goal to determine the amount of anti-D for prevention of active immunization of a D-negative mother giving birth to a D-positive baby. The aim of this study was to evaluate two flowcytometric staining technics for determination of fetal erythrocytes in maternal blood and to recognize the best.

 

 Materials and Methods

 In this experimental study, 34 adult D-negative blood samples were spiked with six serial dilutions of D-positive cord blood (0.125, 0.25, 0.5, 1, 5 and 10%) which was representative of 99.9% of the clinical fetomaternal hemorrhage (FMH) they were stained for flow cytometric analysis. The Fetal Cell Count Kit was used for HbF dual staining and monoclonal anti-D for RhD single staining.

 

 Results

 A comparison between RhD and HbF percentages and FMH volume in spiking samples was performed. A significant correlation between two different parameters in flowcytometric percentages was observed (anti-HbF versus anti-D, r= 0.897, p<0.05). FMH volume was calculated and a significant correlation between expected and observed FMHs in RhD and HbF was obtained (p<0.05, r=0.984, r=0.874). The anti-HbF flowcytometric dual staining allowed better distinction between fetal RBCs (HbF+,CA-), F cells (HbF+,CA+), and adult RBCs (HbF-,CA+). Although Rh-D showed better correlation, but higher values with the RhD in suspensions lower than 10% were noted.

 

 Conclusions

 Data showed that anti-HbF labeling is significantly much more accurate than RhD labeling. Quantification of FMH using these two techniques described allows precise dosage of RhD immunoglobulin for protection against anti-D allo-immunization.

 

Keywords: Hemorrhage, Fetomaternal Transfusion, Flow Cytometry
Full-Text [PDF 385 kb]   (2507 Downloads)    
Type of Study: Research | Subject: Imunology
Published: 2014/07/22
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Khoshnaghsh F, Kheirandish M, Deyhim M. Quantification of fetomaternal hemorrhage: a compariative study of the HbF dual staining and RhD single staining by flow cytometry. Sci J Iran Blood Transfus Organ. 2011; 8 (2) :104-114
URL: http://bloodjournal.ir/article-1-484-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 8, Issue 2 (Summer 2011) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
Persian site map - English site map - Created in 0.04 seconds with 31 queries by YEKTAWEB 4331