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:: Volume 2, Issue 5 (Autumn 2005) ::
Sci J Iran Blood Transfus Organ 2005, 2(5): 139-144 Back to browse issues page
Effects of chimerism on graft-versus-host disease, disease recurrence and survival after HLA-identical marrow transplantation in Iran
H. Ghaffari, K. Alimoghaddam, F. Foroughi , B. Chahardouli, Z. Sanaat, B. Bahar, A. Mousavi, M. Iravani, A. Ghavamzadeh
Abstract:   (29043 Views)

  Abstract

  

 Background and Objectives

  The co-existence of recipient’s and donor’s hematopoietic systems after allogeneic marrow transplantation is called mixed chimerism. Chimerism analysis provides a national method of assessing the ability of different conditioning regimens, graft-versus-host disease (GVHD), prophylactic regimens, and cellular therapy to promote engraftment.

 

  Materials and Methods

  The association of mixed chimerism with acute graft-versus-host disease (GVHD), disease recurrence, survival, and relapse free survival was investigated in 91 patients 12 and 79 of whom underwent either bone or peripheral blood HLA-identical marrow transplantation respectively. Chimerism was assessed using multiplex amplification of shorty tandem repeats (STR-PCR). Cases included thalassemics (19 subjects), AML (29), ALL (20), CML (18) and others (5). Median age was 21 (age range of 3-50). There were 38 females (41.8%) and 53 males (58.2%). Conditioning was busulfan plus cyclophosphamide in 34 patients, busulfan plus fludarabin in 51 patients and busulfan plus fludarabin plus anti-thymocyte globulin in 6 patients. Median time of follow up was 13 months. Data was analyzed using SPSS statistical software.

  

 Results

  On day 30 after transplantation, mixed chimerism (MC) was observed in 15 patients (16.5%), complete donor chimerism (CC) in 72 patients (79%), and no chimerism in 4 patients. The incidence of acute GVHD was significantly lower in mixed chimeras than in complete chimeras (p=0.01) but there was no significant difference in acute GVHD grade (I, II vs. III, IV) between two groups. The incidence of relapse and overall survival were 17.6% and 88.9% respectively showing no significant difference between MC and CC. Relapse free survival was 80.2% and not significantly different between two groups.

 

 Conclusions

  Despite some previous reports, we found no significant difference in survival and relapse rate between MC and CC.

  

  

 Key words: Allogenic, Bone Marrow Transplantation , Chimerism, PCR

Keywords: Allogenic, Bone Marrow Transplantation, Chimerism, PCR
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Type of Study: Research | Subject: General
Published: 2014/06/30
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Ghaffari H, Alimoghaddam K, Foroughi F, Chahardouli B, Sanaat Z, Bahar B, et al . Effects of chimerism on graft-versus-host disease, disease recurrence and survival after HLA-identical marrow transplantation in Iran. Sci J Iran Blood Transfus Organ. 2005; 2 (5) :139-144
URL: http://bloodjournal.ir/article-1-37-en.html


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Volume 2, Issue 5 (Autumn 2005) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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