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:: Volume 20, Issue 3 (َAutumn 2023) ::
Sci J Iran Blood Transfus Organ 2023, 20(3): 179-188 Back to browse issues page
Defensin peptide extraction from leukoreduction filters and determination of minimum inhibitory concentration on Staphylococcus aureus growth
N. Sasani , R. Roghanian , G. Emtiazi , S.M. Jalali , A. Aghaie *
Keywords: Key words: Defensin, Minimum Inhibitory Concentration, Staphylococcus aureus
Full-Text [PDF 465 kb]   (563 Downloads)     |   Abstract (HTML)  (1201 Views)
Type of Study: Research | Subject: Blood transfusion medicine
Published: 2023/09/1
Full-Text:   (437 Views)
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Sci J Iran Blood Transfus Organ 2023;20 (3): 179-188
Orginal Article
 

 Defensin peptide extraction from leukoreduction filters  and determination of minimum inhibitory concentration on Staphylococcus aureus growth

Sasani N.1, Roghanian R.1, Emtiazi G.1, Jalali S.M.2, Aghaie A.3

1Biological Science and Technology, University of Isfahan, Isfahan, Iran
2Iranian Blood Research and Fractionation, Tehran, Iran
3Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran

Abstract
Background and Objectives
Discovery of antibiotics has been one of the important achievements of mankind in the field of infectious diseases, although after a short time, the phenomenon of antibiotic resistance was created. Nowadays, there is a need for an innovation in the control of microbial infections such as the use of antimicrobial peptides. These peptides are abundantly found in neutrophils and since leukoreduction filters are a valuable source of these cells, it has been studied as an available option.

Materials and Methods
In this experimental study, leukoreduction filters was selected by easy non-probability sampling method. First, leukocytes were extracted from leukoreduction filters and then neutrophils were isolated. Defensin peptide was extracted after sonication of neutrophils and was purified by HPLC. Staphylococcus aureus bacteria was cultured and the amount of bacterial inoculation was determined and the minimum inhibitory concentration was measured by macrodilution method. Microbial inhibition halo diameter size of defensin was determined for the intended bacteria.

Results
Using defensin standard, purified defensin peak was collected and the minimum inhibitory concentration was determined as 2.5 μg/mL on Staphylococcus aureus by macrodilution method. Microbial inhibition halo diameter size was calculated as 9 mm.

Conclusions 
The treatment of infectious diseases has faced a challenge with the widespread emergence of drug resistance, and the use of new innovations is inevitable. Defensin as an antimicrobial peptide is a suitable option that is abundantly found in neutrophils obtained from leukoreduction filters and is suggested for research on new antimicrobial drugs.

Key words: Defensin, Minimum Inhibitory Concentration, Staphylococcus aureus




Received: 13 Feb 2023
Accepted:  1 Mar 2023



Correspondence: Aghaie A., PhD of Immunology. Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 82052183; Fax: (+9821) 88601599
E-mail: aghaie.a@gmail.com








 
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Sasani N, Roghanian R, Emtiazi G, Jalali S, Aghaie A. Defensin peptide extraction from leukoreduction filters and determination of minimum inhibitory concentration on Staphylococcus aureus growth. Sci J Iran Blood Transfus Organ 2023; 20 (3) :179-188
URL: http://bloodjournal.ir/article-1-1488-en.html
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Volume 20, Issue 3 (َAutumn 2023) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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