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:: Volume 19, Issue 2 (Summer 2022) ::
Sci J Iran Blood Transfus Organ 2022, 19(2): 148-157 Back to browse issues page
The effect of aqueous and ethanolic extracts of Moringa olifera leaves on inhibition of Jurkat and Raji cell lines of acute lymphoblastic leukemia
L. Rajabi , A. Rajabi , A. Mohammadi , R. Khademi , Gh. Khamisipour
Keywords: Key words: Acute Lymphoblastic Leukemia, Jurkat Cells, Moringa
Full-Text [PDF 509 kb]   (769 Downloads)     |   Abstract (HTML)  (1424 Views)
Type of Study: Research | Subject: Hematology
Published: 2022/07/1
Full-Text:   (1052 Views)
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Sci J Iran Blood Transfus Organ 2022;19 (2): 148-157
Original Article
 





The effect of aqueous and ethanolic extracts of Moringa
olifera leaves on inhibition of Jurkat and Raji cell lines
of acute lymphoblastic leukemia

Rajabi L.1, Rajabi A.2, Mohammadi A.1, Khademi R.3, Khamisipour Gh.1


1Faculty of Para- Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
2Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
3Bushehr Agricultural and Natural Resources Research Center, Bushehr Agricultural Jihad Organization, Bushehr, Iran


Abstract
Background and Objectives
Acute lymphoblastic leukemia is a malignant hematologic disorder of lymphoid lineage. As a result of chemotherapy side effects, treatment with medicinal plants has recently been considered by researchers. Previous studies on Moringa olifera have shown its anti-cancer properties on some cancer cell lines. The aim of this study was to investigate the cytotoxic effects of Moringa oleifera leaves on Jurket and Raji cell lines of acute lymphoblastic leukemia.

Materials and Methods
In this experimental study, after preparing aqueous and ethanolic leaf extract of Moringa olifera, Jurket and Raji cells were treated with different concentrations of extracts for 48 hours. Cell groups’viability was assessed by trypan blue staining and MTT assay. Peripheral blood mononuclear cells (PBMC) were used as a control group. Statistical analysis of data was performed using Gragh Pad Prism 6 and one-way ANOVA test.

Results
Treatment of Jurkat and Raji cells with all concentrations of aqueous and ethanolic extracts led to inhibition of cell growth. The highest percentage of growth inhibition was obtained at a concentration of 150 μg/mL of aqueous extract, 48.5% and 47.4% for Jurkat and Raji cell lines, respectively. Also, the highest lethal percentage was obtained at a concentration of 50 μg/mL of ethanolic extract, 73.4% and 78.5% for Jurkat and Raji cell lines, respectively. The IC50 dose was obtained in all study groups at a concentration of 150 μg/mL.

Conclusions 
The leaves of Moringa olifera have the capacity to inhibit the growth and viability of Jurkat and Raji cell lines of acute lymphoblastic leukemia.

Key words: Acute Lymphoblastic Leukemia, Jurkat Cells, Moringa


Received:  28 Dec 2021
Accepted: 16 Mar 2022



Correspondence: Khamisipour G., PhD in Hematology and Blood Banking, Associate Professor of Faculty of Para- Medicine, Bushehr University of Medical Sciences.
Postal code: 7518759577; Tel: (+9877) 33450178; Fax: (+9877) 33452228
E-mail: khamisipourgholamreza@gmail.com
 
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Rajabi L, Rajabi A, Mohammadi A, Khademi R, Khamisipour G. The effect of aqueous and ethanolic extracts of Moringa olifera leaves on inhibition of Jurkat and Raji cell lines of acute lymphoblastic leukemia. Sci J Iran Blood Transfus Organ 2022; 19 (2) :148-157
URL: http://bloodjournal.ir/article-1-1435-en.html
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Volume 19, Issue 2 (Summer 2022) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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