[Home ] [Archive]   [ فارسی ]  
:: Main :: About us :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 18, Issue 1 (Spring 2021) ::
Sci J Iran Blood Transfus Organ 2021, 18(1): 18-26 Back to browse issues page
Comparison of MTT and Trypan Blue methods in determining the survival of Vero cell line in HSV-1 infection
M. Zamanian, Z. Noormohammadi Dr., T. Akbarzadeh Dr. , F. Bineshian Dr., Z. Sharifi Dr.
Keywords: Key words: HSV-1, Trypan Blue, Survival
Full-Text [PDF 705 kb]   (102 Downloads)     |   Abstract (HTML)  (376 Views)
Type of Study: Research | Subject: Virology
Published: 2021/02/28
Full-Text:   (159 Views)
  1. Ghasemzadeh M, Hosseini E, Ahmadi M, Kamalizad M, Amirizadeh N. Comparable osteogenic capacity of mesenchymal stem or stromal cells derived from human amnion membrane and bone marrow. Cytotechnology 2018; 70(2): 729-39.
  2. Hooshmand K, Asoodeh A. The influence of GL-9 peptide on A549 and human and animal blood cells. Journal of Cellular and Molecular Research (Iranian Journal of Biology) 2017; 29(4): 463-73. [Article in Farsi]
  3. Ziyaeyan M, Alborzi A, Japoni A, Kadivar M, Davarpanah MA, Pourabbas B, et al. Frequency of acyclovir-resistant herpes simplex viruses isolated from the general immunocompetent population and patients with acquired immunodeficiency syndrome. Int J Dermatol 2007; 46(12): 1263-6.
  4. Strober W. Trypan blue exclusion test of cell viability. Curr Protoc Immunol 2001 May;Appendix 3:Appendix 3B. 
  5. Goodwin CJ, Holt SJ, Downes S, Marshall NJ. Microculture tetrazolium assays: a comparison between two new tetrazolium salts, XTT and MTS. J Immunol Methods 1995; 179(1): 95-103. 
  6. Hirst SJ, Barnes PJ, Twort CH. Quantifying proliferation of cultured human and rabbit airway smooth muscle cells in response to serum and platelet-derived growth factor. Am J Respir Cell Mol Biol 1992; 7(6): 574-81.
  7. Ghorbani-Anarkooli M, Dabirian S, Moladoust H, Zendedel A, Bahadori MH. Comparison of MTT, trypan blue, and clonogenic assay, to determine the viability in human anaplastic thyroid cancer cell line. Tehran Univ Med J 2019; 77(1): 26-32. [Article in Farsi]
  8. Bacon TH, Levin MJ, Leary JJ, Sarisky RT, Sutton D. Herpes simplex virus resistance to acyclovir and penciclovir after two decades of antiviral therapy. Clin Microbiol Rev 2003; 16(1): 114-28.
  9. Haggett P, Gunawardena K. Determination of populationthresholds for settlement functions by the Reed-Muench method. The Professional Geographer 1964; 16(4): 6-9.
  10. Strober W. Trypan Blue Exclusion Test of Cell Viability. Curr Protoc Immunol. 2015; 111: A3.B.1-A3.B.3.
  11. Gurr JR, Wang AS, Chen CH, Jan KY. Ultrafine titanium dioxide particles in the absence of photoactivation can induce oxidative damage to human bronchial epithelial cells. Toxicology 2005; 213(1-2): 66-73.
  12. Hosseini F, Sam MR, Jabbari N. Radiosensitivity of radioresistant colorectal cancer cells after treatment with docosahexaenoic acid and irradiation. Tehran University Medical Journal 2014; 72(3): 139-46. [Article in Farsi]
  13. Bellamakondi PK, Godavarthi A, Ibrahim M, Kulkarni S, Naik RM, Maradam S. In vitro cytotoxicity of caralluma species by mtt and trypan blue dye exclusion. Asian Journal of Pharmaceutical and Clinical Research 2014; 7(2): 17-19.
  14. Shokrgozar MA, Zali H, Rezaei Tavirani M, Amanzadeh A. Comparison of MTT and Trypan blue colorimetric methods for determining the cytotoxicity of calprotectin on human stomach cancer cells under laboratory conditions. Kowsar Med J 2007; 12(2): 127-37. [Article in Farsi]
  15. Heidari M, Ghazi-Khansari M, Khoshnoodi J, Shokri F. Comparative measurement of in vitro T-2 toxin cytotoxicity using three different cytotoxicity assays. Toxicology Mechanisms and Methods 2003; 13(2): 153-7.
  16. Fotakis G, Timbrell JA. In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicol Lett 2006; 160(2): 171-7. 

Comparison of MTT and Trypan Blue methods in determining the survival of Vero cell line in HSV-1 infection
Sci J Iran Blood Transfus Organ 2021;18 (1): 18-26
Original Article

Zamanian M.1, Noormohammadi Z.1, Akbarzadeh T.2, Bineshian F.3,
 Sharifi Z.4
1Science and Research Branch, Islamic Azad University, Tehran, Iran
2Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
3Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran
4Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
Background and Objectives
Evaluation of cell survival is important in cellular studies and the result is decisive for the application of the test substance. Different methods are used to evaluate cell survival and the correlation between methods is important. Here, Vero cell line was used to evaluate the effects of HSV-1 virus on cell survival. Cell survival after HSV-1 infection using sensitive colorimetric methods of 3-, 4-, 5-dimethylthiazole-2-yl, -2, 5-diphenyltetrazolium bromide (MTT), and Trypan Blue on Vero cell line was examined.
Materials and Methods
A qualitative study was performed on Vero cell line infected with HSV-1 virus with logarithmic dilutions10-1 to 10-5 dilutions and time intervals of 0, 4, 8, 18 and 24 hours and the s in DMEM containing 2% Fetal bovine serum (FBS). Then cell viability by MTT and Trypan tests was examined . The survival percentage was used to plot the survival curve of the assays.
Different viral dilutions were used to determine the infectious dose of 50% (TCID50) of HSV-1virus with invert microscope. Acyclovir at a concentration of 50 mg/mL was compared as drug control for MTT and Trypan Blue. The results of regression analysis showed cell survival. Linear, positive and significant correlation was observed between the methods that showed a correlation between MTT and Trypan Blue methods (p< 0.001, r = 0.9(
The results showed that both methods can be used to determine cell survival and the results of MTT can be confirmed by Trypan Blue method.
Key words: HSV-1, Trypan Blue, Survival
Received:  11 Nov 2020
Accepted: 30 Jan  2021

Correspondence: Sharifi Z., PhD of Virology. Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.           
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 82052152; Fax: (+9821) 88601555
E-mail: sharifiz@yahoo.com
Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Zamanian M, Noormohammadi Z, Akbarzadeh T, Bineshian F, Sharifi Z. Comparison of MTT and Trypan Blue methods in determining the survival of Vero cell line in HSV-1 infection. Sci J Iran Blood Transfus Organ. 2021; 18 (1) :18-26
URL: http://bloodjournal.ir/article-1-1380-en.html

Volume 18, Issue 1 (Spring 2021) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
Persian site map - English site map - Created in 0.05 seconds with 31 queries by YEKTAWEB 4313