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Comparison of different techniques for loading doxorubicin in platelets microparticles
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A. Darbandi   , F. Yari  , Z. Sharifi , N. Rezaei |
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| Keywords: Doxorubicin, Platelets, Cell-derived microparticles |
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Full-Text [PDF 573 kb]
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| Abstract (HTML) (1715 Views)
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Type of Study: Research |
Subject:
Hematology and Oncology
Published: 2021/02/28
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Full-Text: (1645 Views) |
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Sci J Iran Blood Transfus Organ 2021;18 (1): 60-69
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Comparison of different techniques for loading doxorubicin
in platelets microparticles
Darbandi A.1, Yari F.1, Sharifi Z.1, Rezaie N.2
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
2Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
Abstract
Background and Objectives
Platelet microparticles are microvesicles derived from platelets. Today, with the use of nanoparticles in cancer treatments, many limitations of traditional drug delivery methods are reduced. Doxorubicin, a chemotherapeutic drug available for the treatment of many cancers, has fluorescent properties and can be detected by fluorescent imaging in tissues. Aim of this study is to compare different drug loading methods into platelet microparticles.
Materials and Methods
In this experimental study, the platelet concentrates were taken on their fifth day from Iranian Blood Transfusion Organization. Then platelet microparticles were extracted from those concentrated platelets in several centrifugation stages. Fluorescent microbeads with one-micrometer size and anti-CD41/61 antibody were used to determine the size and identity of microparticles, respectively. Doxorubicin was loaded at 10 µg/ml on platelet microparticles using three methods of incubation, cell-penetrating peptide, and Sonication. Using the auto-fluorescence property of Doxorubicin, the rate of drug loading on platelet microparticles was measured by flow cytometry method.
Results
In terms of size, 95% of the total population of microparticles was less than one micrometer. The expression levels of CD41 and CD61 were 92.39% and 80.03%, respectively. The average fluorescence light intensities calculated in each of the incubation, sonication, and CPP methods were determined to be 79.09% ± 11.37, 47.48% ± 25.12, and 56.69% ± 23.24, respectively.
Conclusions
As the incubation method has higher loading percentage, it could be an effective method for loading drug in platelet microparticles. Furthermore, the use of this method can be considered for clinical studies.
Key words: Doxorubicin, Platelets, Cell-derived microparticles
Received: 19 Sep 2020
Accepted: 21 Sep 2020
Correspondence: Yari F., PhD of Immunology. Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 82052237; Fax: (+9821) 88601555
E-mail: f.yari@ibto.ir |
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Darbandi A, Yari F, Sharifi Z, Rezaei N. Comparison of different techniques for loading doxorubicin in platelets microparticles. Sci J Iran Blood Transfus Organ 2021; 18 (1) :60-69 URL: http://bloodjournal.ir/article-1-1363-en.html
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