[Home ] [Archive]   [ فارسی ]  
:: Main :: About us :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 17, Issue 4 (Winter 2020) ::
Sci J Iran Blood Transfus Organ 2020, 17(4): 270-275 Back to browse issues page
Hepatitis B virus genotyping in asymptomatic HBV blood donors in Tehran
M. Ghanbari Matlob, Z. Sharifi Dr.
Keywords: Hepatitis B virus, Genotype, Blood Donors
Full-Text [PDF 404 kb]   (123 Downloads)     |   Abstract (HTML)  (559 Views)
Type of Study: Research | Subject: Virology
Published: 2020/12/30
Full-Text:   (190 Views)
    References:
 
 
  1. Kao JH, Chen DS. Global control of hepatitis B virus infection. Lancet 2002; 2(7): 395-403.
  2. Tu T, Budzinska MA, Shackel NA, Urban S. HBV DNA Integration: Molecular Mechanisms and Clinical Implications. Viruses 2017; 9(4): 75.
  3. Poustchi H, Mohamadkhani A, Bowden S, Montazeri G, Ayres A, Revill P, et al. Clinical significance of precore and core promoter mutations in genotype D hepatitis B-related chronic liver disease. J Viral Hepat 2008; 15(10): 753-60.
  4. Altaf A, Shah SA, Zaidi NA, Memon A, Nadeem ur R, Wray N. High risk behaviors of injection drug users registered with harm reduction programme in Karachi, Pakistan. Harm Reduct J 2007; 4(1): 7.
  5. Nodeh MM, Mosavat A, Valizadeh N, Zadeh AM, Boskabadi A, Mashkani B, et al. Genotype characteristic and phylogenetic analysis of hepatitis B virus in northeast-Iran. Infect Genet Evol 2018; 59: 148-54.
  6. Kidd-Ljunggren K, Miyakawa Y, Kidd AH. Genetic variability in hepatitis B viruses. J Gen Virol 2002; 83(Pt 6): 1267-80.
  7. Pettersson JH, Myking S, Elshaug H, Bygdas KIE, Stene-Johansen K. Molecular epidemiology of hepatitis B virus infection in Norway. BMC Infect Dis 2019;
    19(1): 236.
  8. Kheirabad AK, Farshidfar G, Nasrollaheian S, Gouklani H. Prevalence and Characteristics of Precore Mutation in Iran and Its Correlation with Genotypes of Hepatitis B. Electronic Physician 2017; 9(4): 4114.
  9. Pourkarim MR, Sharifi Z, Soleimani A, Amini-Bavil-Olyaee S, Elsadek Fakhr A, Sijmons S, et al. Evolutionary analysis of HBV “S” antigen genetic diversity in Iranian blood donors: a nationwide study. J Med Virol 2014; 86(1): 144-55.
  10. Kramvis A, Kew M, François G. Hepatitis B virus genotypes. Vaccine 2005; 23(19): 2409-23.
  11. Lin CL, Kao JH. The clinical implications of hepatitis B virus genotype: Recent advances. J Gastroenterol Hepatol. 2011;26 Suppl 1:123-30.
  12. Attaran MS, Hosseini SM, Fakhari J, Sharifi Z. Serological and molecular characterization of hepatitis B virus in asymptomatic blood donors in Iran. Iran J Microbiol 2018; 10(1): 59-64.
  13. Mozhgani SH, Malekpour SA, Norouzi M, Ramezani F, Rezaee SA, Poortahmasebi V, et al. Molecular evolution and phylodynamics of hepatitis B virus infection circulating in Iran. Arch Virol 2018; 163(6): 1479-88.
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

 
 
 
 
Sci J Iran Blood Transfus Organ 2020;17 (4): 270-275
Original Article
 
 

Hepatitis B virus genotyping in asymptomatic HBV blood donors in Tehran
 
Ghanbari Matloob M.1, Sharifi Z.1
 
 
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
 
 
Abstract
Background and Objectives
Hepatitis B virus (HBV) infection remains a serious global health challenge. Hepatitis B infection is one of the major diseases transmitted by blood transfusion. Infection with the virus can cause extensive liver damage.  So far, 10 genotypes have been identified for hepatitis B. The aim of this study was to do Hepatitis B virus genotyping in asymptomatic HBV blood donors in Tehran.
 
Materials and Methods
In this cross-sectional study, 20 HBsAg positive blood donors tested by ELISA were examined. After viral DNA extraction, the pres gene was amplified by Nested-PCR method and the virus genotype was aligned with the references sequence by Clustal w software.
 
Results
Samples were sequenced after Nested-PCR was done. Hepatitis B virus genotype was determined in all 20 blood donors with hepatitis B to be genotype D.
 
Conclusions 
Prevalence of genotype D in study subjects was 100%.  This result can be used in preparation of hepatitis B diagnostic kits. Identifying genotypes can also help physicians identify patients at higher risk for disease progression and adopt better treatment strategies.
 
Key words: Hepatitis B virus, Genotype, Blood Donors
 
 
 
 
 
 
 
 
 
 
 
 
 
Received:  12 May 2020
Accepted: 12 Jul   2020
 
 

Correspondence: Sharifi Z., PhD of Virology. Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.           
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 82052152; Fax: (+9821) 88601555
E-mail: sharifiz@yahoo.com
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ghanbari Matlob M, Sharifi Z. Hepatitis B virus genotyping in asymptomatic HBV blood donors in Tehran. Sci J Iran Blood Transfus Organ. 2020; 17 (4) :270-275
URL: http://bloodjournal.ir/article-1-1349-en.html


Volume 17, Issue 4 (Winter 2020) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
Persian site map - English site map - Created in 0.05 seconds with 31 queries by YEKTAWEB 4313