|
Evaluation of mitochondrial DNA during platelet storage prepared by platelet rich plasma
|
L. Vakili   , A. Chegini  , Sh. Samiee , M. Shaiegan |
|
|
| Keywords: Real-Time Polymerase Chain Reaction, DNA, Platelet-Rich Plasma |
|
|
Full-Text [PDF 451 kb]
(559 Downloads)
| Abstract (HTML) (1588 Views)
|
Type of Study: Research |
Subject:
Blood Transfusion
Published: 2021/02/28
|
|
|
|
|
|
|
Full-Text: (1270 Views) |
References:
- Yasui K, Matsuyama N, Kuroishi A, Tani Y, Furuta RA, Hirayama F. Mitochondrial damage-associated molecular patterns as potential proinflammatory mediators in post–platelet transfusion adverse effects. Transfusion 2016; 56(5): 1201-12.
- Zadsar M, Naserani Pour M, Chegini A, Lotfi A, Mojtabavi Naeeni M. Platelet consumption, indications, transfusion triggers, and response to treatment in hospital wards in Tehran. Sci J Iran Blood Transfus Organ 2015; 12(2): 163-72. [Article in Farsi]
- Hod E, Schwartz J. Platelet transfusion refractoriness. Br J Haematol 2008; 142(3): 348-60.
- Land WG. Transfusion-related acute lung injury: the work of DAMPs. Transfus Med Hemother 2013; 40(1): 3-13.
- Simmons JD, Lee YL, Pastukh VM, Capley G, Muscat CA, Muscat DC, et al. Potential contribution of mitochondrial (mt) DNA damage associated molecular patterns (DAMPs) in transfusion products to the development of acute respiratory distress syndrome (ARDS) after multiple transfusions. J Trauma Acute Care Surg 2017; 82(6): 1023-9.
- Schwartz RS, Conley CL. Blood. In: Encyclopedia Britannica. Available from: https:// www. britannica.com/science/blood-biochemistry/additional-info#contributors.
- Baaten CCFMJ, Moenen FCJI, Henskens YMC, Swieringa F, Wetzels RJH, van Oerle R, et al. Impaired mitochondrial activity explains platelet dysfunction in thrombocytopenic cancer patients undergoing chemotherapy. Haematologica 2018; 103(9): 1557–67.
- Vénéreau E, Ceriotti C, Bianchi ME. DAMPs from cell death to new life. Front Immunol 2015; 6: 422.
- Boudreau LH, Duchez AC, Cloutier N, Soulet D, Martin N, Bollinger J, et al. Platelets release mitochondria serving as substrate for bactericidal group IIA-secreted phospholipase A2 to promote inflammation. Blood 2014; 124(14): 2173-83.
- Cognasse F, Payrat JM, Corash L, Osselaer JC, Garraud O. Platelet components associated with acute transfusion reactions: the role of platelet-derived soluble CD40 ligand. Blood 2008; 112(12): 4779-80.
- Chiu RW, Chan LY, Lam NY, Tsui NB, Ng EK, Rainer TH, et al. Quantitative analysis of circulating mitochondrial DNA in plasma. Clin Chem 2003; 49(5): 719-26.
- Bakkour S, Acker JP, Chafets DM, Inglis HC, Norris PJ, Lee TH, Busch MP. Manufacturing method affects mitochondrial DNA release and extracellular vesicle composition in stored red blood cells. Vox Sang 2016; 111(1): 22-32.
- Hotz MJ, Qing D, Shashaty MG, Zhang P, Faust H, Sondheimer N, et al. Red blood cells homeostatically bind mitochondrial DNA through TLR9 to maintain quiescence and to prevent lung injury. Am J Respir Crit Care Med 2018; 197(4): 470-80.
- Lauring AS, Lee T-H, Martin JN, Hunt PW, Deeks SG, Busch M. Lack of evidence for mtDNA as a biomarker of innate immune activation in HIV infection. PLoS One 2012; 7(11): e50486.
- Lee YL, King MB, Gonzalez RP, Brevard SB, Frotan MA, Gillespie MN, et al. Blood transfusion products
contain mitochondrial DNA damage-associated molecular patterns: a potential effector of transfusion-related acute lung injury. J Surg Res 2014; 191(2): 286-9.
- Kaufman RM, Assmann SF, Triulzi DJ, Strauss RG, Ness P, Granger S, et al. Transfusion-related adverse events in the P latelet D ose study. Transfusion 2015; 55(1): 144-53.
- Tung JP, Fraser JF, Nataatmadja M, Colebourne KI, Barnett AG, Glenister KM, et al. Age of blood andrecipient factors determine the severity of transfusion-related acute lung injury (TRALI). Crit Care 2012; 16(1): R19.
- Heddle NM. Pathophysiology of febrile nonhemolytic trans-fusion reactions. Curr Opin Hematol 1999; 6(6): 420-6.
- Sayah DM, Looney MR, Toy P. Transfusion reactions: newerconcepts on the pathophysiology, incidence, treatment, andprevention of transfusion-related acute lung injury. Crit Care Clin 2012; 28(3): 363-72.
- Cognasse F, Aloui C, Anh Nguyen K, Hamzeh-Cognasse H, Fagan J, Arthaud CA, et al. Platelet components associated with adverse reactions: predictive value of mitochondrial DNA relative to biological response modifiers. Transfusion 2016; 56(2): 497-504.
|
Sci J Iran Blood Transfus Organ 2021;18 (1): 27-35
|
Evaluation of mitochondrial DNA during platelet storage prepared by platelet rich plasma
Vakili L.1, Chegini A.1, Samiei Sh.1, Shaiegan M.1
2Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
Abstract
Background and Objectives
Platelet activation occurs for a variety of reasons from the moment of sampling to that of injection. Activation of the platelets leads to activation of mitochondria. They move toward the membrane, sprouting and shedding freely from it, leading to the release of pro inflammatory mediators and mtDNA. Therefore, mtDNA level is considered as one of the parameters of platelet activity. The aim of this study was to compare mtDNA levels during storage.
Materials and Methods
This experimental study was performed on 22 PRP products. We obtained consent of all donors referring to Iran Innovation Center of Iranian Blood Transfusion Organization. Samples were taken from the PRP bags on day 1, 3 and 5. Then, the fold change of mtDNA was assessed by Real time PCR. The results were analyzed by paired t-test and the P value of less than 0.05 was considered significant.
Results
The rate of mtDNA decreased 0.8-fold on day 3 to 1, 0.6-fold on day 5 to 3, and 0 .49 on day 5 to 1. These changes were significant on days 5 to 1 (p= 0.003) and they were not significant on days 3 to 1 (p= 0.4).
Conclusions
The amount of mtDNA increased on the first day, and then decreased during the storage of PRP products.
Key words: Real-Time Polymerase Chain Reaction, DNA, Platelet-Rich Plasma
Received: 2 Jun 2020
Accepted: 9 Feb 2021
Correspondence: Chegini A., MD. Specialist in Anesthesiology. Assistant Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 82052256; Fax: (+9821) 88601599
E-mail: a.chegini@ibto.ir |
|
| Send email to the article author |
|
|
|
| Add your comments about this article |
|
|
|
|
Vakili L, Chegini A, Samiee S, Shaiegan M. Evaluation of mitochondrial DNA during platelet storage prepared by platelet rich plasma. Sci J Iran Blood Transfus Organ 2021; 18 (1) :27-35 URL: http://bloodjournal.ir/article-1-1334-en.html
|
.jpg)
.png)
