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:: Volume 18, Issue 1 (Spring 2021) ::
Sci J Iran Blood Transfus Organ 2021, 18(1): 27-35 Back to browse issues page
Evaluation of mitochondrial DNA during platelet storage prepared by platelet rich plasma
L. Vakili , A. Chegini ِDr., Sh. Samiee, M. Shaiegan Dr.
Keywords: Real-Time Polymerase Chain Reaction, DNA, Platelet-Rich Plasma
Full-Text [PDF 451 kb]   (97 Downloads)     |   Abstract (HTML)  (406 Views)
Type of Study: Research | Subject: Blood Transfusion
Published: 2021/02/28
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Sci J Iran Blood Transfus Organ 2021;18 (1): 27-35
Original Article
 
 

Evaluation of mitochondrial DNA during platelet storage prepared by platelet rich plasma
 
Vakili L.1, Chegini A.1, Samiei Sh.1, Shaiegan M.1
 
 
 
2Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
 
 
 
Abstract
Background and Objectives
Platelet activation occurs for a variety of reasons from the moment of sampling to that of injection. Activation of the platelets leads to activation of mitochondria. They move toward the membrane, sprouting and shedding freely from it, leading to the release of pro inflammatory mediators and mtDNA. Therefore, mtDNA level is considered as one of the parameters of platelet activity. The aim of this study was to compare mtDNA levels during storage.
 
Materials and Methods
This experimental study was performed on 22 PRP products. We obtained consent of all donors referring to Iran Innovation Center of Iranian Blood Transfusion Organization. Samples were taken from the PRP bags on day 1, 3 and 5. Then, the fold change of mtDNA was assessed by Real time PCR. The results were analyzed by paired t-test and the P value of less than 0.05 was considered significant.
 
Results
The rate of mtDNA decreased 0.8-fold on day 3 to 1, 0.6-fold on day 5 to 3, and 0 .49 on day 5 to 1. These changes were significant on days 5 to 1 (p= 0.003) and they were not significant on days 3 to 1 (p= 0.4).
 
Conclusions 
The amount of mtDNA increased on the first day, and then decreased during the storage of  PRP products.
 
Key words: Real-Time Polymerase Chain Reaction, DNA, Platelet-Rich Plasma
 
 
 
 
 
 
 
 
 
Received: 2 Jun  2020
Accepted: 9 Feb 2021
 
 

Correspondence: Chegini A., MD. Specialist in Anesthesiology. Assistant Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 82052256; Fax: (+9821) 88601599
E-mail: a.chegini@ibto.ir
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Vakili L, Chegini A, Samiee S, Shaiegan M. Evaluation of mitochondrial DNA during platelet storage prepared by platelet rich plasma. Sci J Iran Blood Transfus Organ. 2021; 18 (1) :27-35
URL: http://bloodjournal.ir/article-1-1334-en.html


Volume 18, Issue 1 (Spring 2021) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
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