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Determination of fucosyltransferase 3 (FUT3) gene polymorphisms frequency in Iranian blood donors
 
Naseri Rad M.¹, Mardani A.¹, Naghi A.A.¹, Shahabi M.^1
 
 
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
 
 
Abstract
Background and Objectives
The FUT3 gene regulates the expression of Lewis blood group antigens mainly L^ea and L^eb. The Lewis negative phenotype, is the result of an inactivated FUT3 enzyme that lacks glycosidase activity. Several single nucleotide polymorphisms (SNPs) may cause enzyme inactivation with different racial distribution. This study aimed to determine the frequency of these SNPs in Iranian blood donors.
 
Materials and Methods
In this cross sectional descriptive study, one hundred blood donors from Tehran Blood Transfusion Center were analyzed. Genomic DNA was extracted and the coding sequence of FUT3 gene was amplified with specific primers. PCR products were directly sequenced. Haplotypes were inferred by SNP Analyzer software.
 
Results
A total of 15 SNPs including 10 nonsense SNPs were recognized. The frequency of most common SNPs was detected as 39%(202T>C), 37%(314C>T), 20%(59T>G), 9%(508G>A), and 8%(47G>C). Haplotype analysis revealed 13 haplotypes. The frequency of major haplotypes was detected as 66.2%(Le), 17.5%(le^202,314), 4.3 %(le^47,202,314), 4.1 %(le⁵⁹), and 3.4%(le^59,508).
 
Conclusions 
Our findings indicate that 202T > C, 59T > G and 508G > A are useful as major SNPs for detection of Lewis-negative alleles in genotyping of Lewis blood groups; they can also play an effective role in large-scale studies associated with diseases in our population.
 
Key words: SNPs, Haplotypes, Alleles
 
 
 
 
 
 
 
 
Received:   8  Oct 2019
Accepted: 24 Jun 2020
 
 

Correspondence: Shahabi M., PhD of Biological Science. Assistant professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 88601558; Fax: (+9821) 88601542
E-mail: majshahabi@yahoo.com