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:: Volume 17, Issue 1 (Spring 2020) ::
Sci J Iran Blood Transfus Organ 2020, 17(1): 1-11 Back to browse issues page
Update on HCV genotypes among Iranian blood donors
F. Ranjbar Kermani Dr., S. Amini Kafi Abad Dr. , K. Mousavi Hosseini Dr., M. Maghsudlu Dr., Z. Sharifi Dr.
Keywords: Hepatitis C, Genotype, Blood Donors
Full-Text [PDF 509 kb]   (315 Downloads)     |   Abstract (HTML)  (2054 Views)
Type of Study: Research | Subject: Blood transfusion medicine
Published: 2020/03/16
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Sci J Iran Blood Transfus Organ 2020;17(1): 1-11
Original Article
 

 

Update on HCV genotypes among Iranian blood donors
 
Ranjbar Kermani F.1, Amini Kafi-Abad S.1, Mousavi Hosseini K.1, Maghsudlu M.1, Sharifi Z.1
 
 
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
 
 
Abstract
Background and Objectives
Hepatitis C (HCV) infection is one of the main causes of chronic hepatitis diseases all over the world. HCV is a transfusion transmitted virus and a serious threat to general health. HCV genotyping has an important role in tracing routes of infection. This study aimed at investigating the changes in  distribution pattern of HCV genotypes among Iranian blood donors.
 
Materials and Methods
In this cross sectional study, 239 HCV confirmed blood donors from Jun. 2015 to Nov. 2017 were included. Semi nested PCR method was used for amplifying the NS5B  region of HCV genome. PCR products were sequenced and HCVgenotypes were determined by searching the sequences in Basic Local Alignment Search Tool (BLAST). Phylogenetic tree was constructed to confirm HCV genotypes. STATA 13 software was used for data analysis. 
 
Results
In 106 (44.35% ± 6.3%) out of 239 participants, HCV genotype was determined and confirmed. In phylogenetic tree, studied sequences formed three separated clusters. Genotype 3a, 1a and 1b were the common genotypes.
 
Conclusions 
It seems that molecular epidemiology of HCV infection did not change based on variability of genotypes but changes in the frequency of genotypes have been occurred as a result of replacement of genotype 1a by genotype 3a during the last decade among Iranian blood donors.
 
Key words: Hepatitis C, Genotype, Blood Donors
 
 
 
 
 
 
 
 
 
 
Received:  25 Aug 2019
Accepted: 23 Nov 2019
 
 

Correspondence: Amini Kafi-Abad S., MD. Pathologist. Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 88601558; Fax: (+9821) 88601542
E-mail: s.amini@ibto.ir
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Ranjbar Kermani F, Amini Kafi Abad S, Mousavi Hosseini K, Maghsudlu M, Sharifi Z. Update on HCV genotypes among Iranian blood donors. Sci J Iran Blood Transfus Organ. 2020; 17 (1) :1-11
URL: http://bloodjournal.ir/article-1-1299-en.html


Volume 17, Issue 1 (Spring 2020) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
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